pBOMB4-Tet-mCherry vector (V009113)

LOCUS       V009113                12003 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009113
VERSION     V009113
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Bauler LD, Hackstadt T.
  TITLE     Expression and Targeting of Secreted Proteins from Chlamydia
            trachomatis
  JOURNAL   J. Bacteriol. 196 (7), 1325-1334 (2014)
   PUBMED   24443531
REFERENCE   2  (bases 1 to 12003)
  AUTHORS   Bauler LD, Hackstadt T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2013) Laboratory of Intracellular Parasites, Rocky
            Mountain Laboratories, NIAID, NIH, 903 S. Fourth St., Hamilton, MT
            59840, USA
REFERENCE   3  (bases 1 to 12003)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12003)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2014"; volume: "196"; issue: "7"; pages:
            "1325-1334"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-OCT-2013) Laboratory of Intracellular Parasites, Rocky Mountain
            Laboratories, NIAID, NIH, 903 S. Fourth St., Hamilton, MT 59840,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(55..969)
                     /note="Virulence plasmid integrase pGP7-D from Chlamydia
                     trachomatis. Accession#: P0CE19"
                     /label="Virulence plasmid integrase pGP7-D"
     CDS             complement(7570..8193)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     promoter        8209..8264
                     /label="tetR/tetA promoters"
                     /note="overlapping promoters for bacterial tetR and tetA"
     RBS             8282..8293
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             8308..9015
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     promoter        9037..9108
                     /label="AmpR promoter"
     CDS             9109..9966
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      10138..10722
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             11262..11975
                     /label="GFP (S65T)"
                     /note="S65T variant of Aequorea victoria green fluorescent
                     protein (Heim et al., 1995)"