pBMTL-6 vector (V009116)

LOCUS       40924_6956        5922 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBMTL-6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Lynch MD, Gill RT.
  TITLE     Broad host range vectors for stable genomic library construction
  JOURNAL   Biotechnol. Bioeng. 94 (1), 151-158 (2006)
  PUBMED    16496398
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Lynch MD, Gill RT.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2005) Chemical and Biological Engineering, 
            University of Colorado @ Boulder, ECCH 111, Campus Box 424, Boulder,
            CO 80309, USA
REFERENCE   3  (bases 1 to 5922)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5922)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng."; date: "2006"; volume: "94"; issue: "1"; pages: "151-158"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JUN-2005) Chemical and Biological Engineering, University of 
            Colorado @ Boulder, ECCH 111, Campus Box 424, Boulder, CO 80309, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(60..96)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     CDS             complement(104..1123)
                     /codon_start=1
                     /gene="mob"
                     /product="Mob"
                     /label=mob
                     /note="required for mobilization"
                     /protein_id="AAZ83202.1"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSKKPLLS"
     gene            complement(104..1123)
                     /gene="mob"
                     /label=mob
                     /note="derived from pBBR1MCS in GenBank Accession Number
                     U02374"
     rep_origin      1347..2116
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             2117..2776
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     CDS             2876..3649
                     /codon_start=1
                     /gene="kilA"
                     /product="KilA"
                     /label=kilA
                     /note="involved in tellurite resistance"
                     /protein_id="AAZ83204.1"
                     /translation="MEEQSVNMARLKGEVLPALFASPATIGEYGAGIDGADSLNELSNL
                     MEHGAVAALADKISQIVAKLADADPRKIAEKPTWFEKMLGREVERQVRYQVARKTLDQL
                     LDEAEGVAQRVRDTLRALDDMLNTHEAEVDRLRAYIQAGREFLDENPEAGAAKAGVIEF
                     DKPRERFARKLANLATLMASHEMSVTQMKLTRAQAVDMLDRFSETASVLVPVWRQHTLA
                     LITTKNMNPAMVAEAAKAHQALMRSLSQSLEGINQ"
     gene            2876..3649
                     /gene="kilA"
                     /label=kilA
     CDS             3667..4803
                     /codon_start=1
                     /gene="telA"
                     /product="TelA"
                     /label=telA
                     /note="involved in tellurite resistance"
                     /protein_id="AAZ83205.1"
                     /translation="MNALKTTHDAKAPIVAFDMTPATLRELGLQESDVPEVHAVAQRIE
                     VGSPQTVAEFGRDVAEHTSRYADSLLDQVRNSDLDEAGEKLTQVVAKARSLNVGPLSDN
                     RSRLPLIGPLIDRFRVRSTGFMARFDTTREQIEHLVSEVQTTQQGIAQRNASLDEMFAA
                     VREEHRLLGVHIAAGKVRLAELREQAEGLRGNVGNDPGRVQELADLDAMVANLDKRIGD
                     LIALQHSAMQSLPTIRMIQANNQMLVDKFHTIREITVPAWKRQFMLALSLNEQKNAVEL
                     ATAIDDTTNDLMKRNAALLHRTSVETAKENQRLVIDVDTLKQVQTTLIKTVEDVIRIQQ
                     EGVQKRKDAEKQIAAMRGDLQAKLTRQPVRELAQQESV"
     gene            3667..4803
                     /gene="telA"
                     /label=telA
     CDS             4800..5753
                     /codon_start=1
                     /gene="telB"
                     /product="TelB"
                     /label=telB
                     /note="involved in tellurite resistance"
                     /protein_id="AAZ83206.1"
                     /translation="MNATNTDVFAQVGGLEARGAKMKKRGTRFLIAALAVLAIAGIGAV
                     TGWAISPSATPGSIDVPQVLASTFSDQVPGSEGGGLGGGLPFTSAVGAFTDFMAGPAIF
                     TLGILGIVVAGAVLVFGGEFCGFVRSVCMMVIAVSMIFVSSNLVKGILGGDHDAGPAEP
                     SPRARFMAAVEAKDFARVQELIEARGAKSAADYVLAQLAVAEGLDRKPGARVVVGKAAG
                     SMAMPPAALGFTPRGEAAYAIERSAYGEPRSSIAKQYQQEWNRKAATWWAMAGVAGIIG
                     AILAAAATGFVGLAVSIRNRVKRVRDLLVMEPGAEP"
     gene            4800..5753
                     /gene="telB"
                     /label=telB
     terminator      complement(5767..5798)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        5845..5875
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5883..5899
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."