pBMPGal4LWL vector (V009140)

LOCUS       40924_6836       11213 bp DNA     circular SYN 17-DEC-2018
DEFINITION  InSITE enhancer fusion vector pBMPGal4LWL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11213)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR.
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns
  JOURNAL   Nat. Methods 8 (3), 231-237 (2011)
  PUBMED    21473015
REFERENCE   2  (bases 1 to 11213)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin C-C., Potter 
            CJ, Clandinin TR.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2011) Neurobiology, Stanford University, 299 W. 
            Campus Dr., Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 11213)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11213)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods"; date: "2011"; volume: "8"; issue: "3"; pages: "231-237"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-JAN-2011) Neurobiology, Stanford University, 299 W. Campus Dr., 
            Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    689..758
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     primer_bind     949..965
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1096..1195
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     regulatory      1276..1382
                     /label=P transposase promoter
                     /note="P transposase promoter"
                     /regulatory_class="promoter"
     CDS             1458..4100
                     /label=GAL4
                     /note="GAL4 transcriptional activator"
     3'UTR           4348..4615
                     /label=hsp70 3'UTR
                     /note="hsp70 3'UTR"
     primer_bind     complement(4599..4615)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     4633..4666
                     /label=loxP
                     /note="loxP"
     protein_bind    4633..4666
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     gene            4842..8961
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    complement(8967..9000)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(9025..9043)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9064..9080)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9088..9104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9112..9142)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9157..9178)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9466..10054)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10228..11085)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(11086..11190)
                     /label=AmpR promoter