pBIOTRX-BirA vector (V009179)

LOCUS       40924_6541        4610 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBIOTRX-BirA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   LaVallie ER, DiBlasio EA, Kovacic S, Grant KL, Schendel PF, McCoy 
            JM.
  TITLE     A thioredoxin gene fusion expression system that circumvents 
            inclusion body formation in the E. coli cytoplasm
  JOURNAL   Biotechnology (N.Y.) 11 (2), 187-193 (1993)
  PUBMED    7763371
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Smith PA, Tripp BC, DiBlasio-Smith EA, Lu Z, LaVallie ER, McCoy JM.
  TITLE     A plasmid expression system for quantitative in vivo biotinylation 
            of thioredoxin fusion proteins in Escherichia coli
  JOURNAL   Nucleic Acids Res. 26 (6), 1414-1420 (1998)
  PUBMED    9490786
REFERENCE   3  (bases 1 to 4610)
  AUTHORS   Smith PA, Tripp BC, DiBlasio-Smith EA, Lu Z, LaVallie ER, McCoy JM.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-1998) Molecular Biology and Gene Expression, 
            Genetics Institute, Inc., 87 Cambridge Park Drive, Cambridge, MA 
            02140, USA
REFERENCE   4  (bases 1 to 4610)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4610)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechnology (N.Y.)"; date: "1993"; volume: "11"; issue: "2"; 
            pages: "187-193"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1998"; volume: "26"; issue: "6"; pages: 
            "1414-1420"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-1998) Molecular Biology and Gene Expression, Genetics 
            Institute, Inc., 87 Cambridge Park Drive, Cambridge, MA 02140, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      2159..2187
                     /label=lambda pL
                     /note="lambda pL"
                     /regulatory_class="promoter"
     CDS             2416..2481
                     /label=lambda N peptide
                     /note="N-terminal RNA-binding domain from the lambda
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
     misc_feature    2724..2792
                     /note="Tag 81; biotinylation recognition sequence"
     CDS             2793..3119
                     /label=TrxA
                     /note="E. coli thioredoxin"
     CDS             3154..4116
                     /label=BirA
                     /note="E. coli biotin protein ligase"
     regulatory      4138..4204
                     /label=AspA gene transcription terminator
                     /note="AspA gene transcription terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(4214..4230)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"