Basic Vector Information
- Vector Name:
- pLenti-MP2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6782 bp
- Type:
- Lentiviral
- Replication origin:
- ori
- Promoter:
- RSV
pLenti-MP2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLenti-MP2 vector Sequence
LOCUS pLenti-MP2. 6782 bp DNA circular SYN 13-MAY-2021
DEFINITION 3rd generation lentiviral plasmid.
ACCESSION .
VERSION .
KEYWORDS pLenti-MP2.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6782)
AUTHORS Enomoto M, Bunge MB, Tsoulfas P
TITLE A multifunctional neurotrophin with reduced affinity to p75NTR
enhances transplanted Schwann cell survival and axon growth after
spinal cord injury.
JOURNAL Exp Neurol. 2013 Oct;248:170-82. doi:
10.1016/j.expneurol.2013.06.013. Epub 2013 Jun 20.
PUBMED 23792206
REFERENCE 2 (bases 1 to 6782)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 6782)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Exp
Neurol."; date: "2013-10"; pages: "
10.1016/j.expneurol.2013.06.013. Epub 2013 Jun 20"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6782
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 3..229
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 230..410
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 457..582
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1075..1308
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1493..1537
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1686..1727
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
misc_feature 1804..1920
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 1943..2246
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2247..2450
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2447..2471
/label=LNCX
/note="Human CMV promoter, forward primer"
misc_feature 2628..3216
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 3303..3536
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 3608..3742
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 3769..3904
/label=SV40 ori
/note="SV40 origin of replication"
promoter complement(3925..3943)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3953..3969)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 4111..4566
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4592..4696
/label=AmpR promoter
CDS 4697..5554
/label=AmpR
/note="beta-lactamase"
rep_origin 5728..6316
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6470..6487
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 6604..6625
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6640..6670
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6678..6694
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6702..6718
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 6739..6757
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
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