HA-HIF1alpha-wt-pBabe-puro vector (V010534)

MSDS

Basic Vector Information

Vector Name:: HA-HIF1alpha-wt-pBabe-puro

Antibiotic Resistance:: Ampicillin

Length:: 7623 bp

Type:: Mammalian Expression, Retroviral

Replication origin:: ori

Selection Marker:: Puromycin

Copy Number:: High Copy

Cloning Method:: Restriction Enzyme

5' Primer:: pBabe-5'

HA-HIF1alpha-wt-pBabe-puro vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

HA-HIF1alpha-wt-pBabe-puro vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       HA-HIF1alpha-wt-        7623 bp DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    HA-HIF1alpha-wt-pBabe-puro.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Yan Q, Bartz S, Mao M, Li L, Kaelin WG
  TITLE     The hypoxia-inducible factor 2alpha N-terminal and C-terminal 
            transactivation domains cooperate to promote renal tumorigenesis in 
            vivo.
  JOURNAL   Mol Cell Biol. 2007 Mar . 27(6):2092-102.
  PUBMED    17220275
REFERENCE   2  (bases 1 to 7623)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7623)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Mar . 27(6):2092-102."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    530..729
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             930..1346
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     CDS             1378..1404
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1423..3900
                     /gene="HIF1A"
                     /label=HIF1A
                     /note="Hypoxia-inducible factor 1-alpha from Homo sapiens. 
                     Accession#: Q16665"
     promoter        3946..4275
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4285..4881
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     LTR             4983..5574
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     primer_bind     complement(5704..5721)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5875..6463)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6626..7483)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7484..7588)
                     /label=AmpR promoter

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