pAAV-tTS vector (V009662)

LOCUS       40924_3209        7993 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAAV-tTS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Chen Y, Cao L, Luo C, Ditzel DA, Peter J, Sprengel R.
  TITLE     RANGE: Gene Transfer of Reversibly Controlled Polycistronic Genes
  JOURNAL   Mol Ther Nucleic Acids 2, E85 (2013)
  PUBMED    23571608
REFERENCE   2  (bases 1 to 7993)
  AUTHORS   Chen Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2012) Molecular Neurobiology, Max Planck Institute
            for Medical Research, Jahnstr. 29, Heidelberg, BW 69120, Germany
REFERENCE   3  (bases 1 to 7993)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7993)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids 2, E85 (2013)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-MAY-2012) Molecular Neurobiology, Max Planck Institute for 
            Medical Research, Jahnstr. 29, Heidelberg, BW 69120, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   19..163
                     /rpt_family="AAV2 ITR"
     polyA_signal    248..296
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        371..818
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             827..2476
                     /label=luciferase
                     /note="firefly luciferase"
     mat_peptide     2480..2533
                     /product="2A peptide"
                     /note="self-cleaving"
     CDS             2480..2533
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2546..3391
                     /label=tTS
                     /note="fusion of a hybrid tetracycline repressor TetR with
                     a nuclear localization signal and a KRAB silencing domain"
     mat_peptide     3395..3448
                     /product="2A peptide"
     CDS             3395..3448
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3461..4114
                     /label=hmKO
                     /note="humanized monomeric Kusabira-Orange fluorescent
                     protein"
     misc_feature    4150..4738
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    4780..4987
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4996..5140
                     /rpt_family="AAV2 ITR"
     rep_origin      5239..5694
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6026..6130
                     /label=AmpR promoter
     CDS             6131..6988
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7162..7750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"