pA1-Agc-bgyg-3'N vector (V009692)

LOCUS       40924_2977       11068 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pA1-Agc-bgyg-3'N DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11068)
  AUTHORS   Ikeda Y, Ito K, Izumi Y, Shinomura T.
  TITLE     A candidate enhancer element responsible for high-level expression 
            of the aggrecan gene in chondrocytes
  JOURNAL   J. Biochem. 156 (1), 21-28 (2014)
  PUBMED    24554731
REFERENCE   2  (bases 1 to 11068)
  AUTHORS   Ikeda Y, Shinomura T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2013) Contact:Yuichi Ikeda Tokyo Medical and 
            Dental University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, 
            Tokyo 113-8549, Japan URL :http://www.tmd.ac.jp/
REFERENCE   3  (bases 1 to 11068)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11068)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biochem."; date: "2014"; volume: "156"; issue: "1"; pages: "21-28"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-AUG-2013) Contact:Yuichi Ikeda Tokyo Medical and Dental 
            University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, Tokyo 
            113-8549, Japan URL :http://www.tmd.ac.jp/"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_binding    207..565
                     /label=SOX9 binding site
                     /bound_moiety="SOX9"
                     /note="A1"
     regulatory      704..1237
                     /gene="bgyg"
                     /label=rat Agc1 promoter sequence
                     /note="rat Agc1 promoter sequence"
                     /regulatory_class="promoter"
     exon            1238..1618
                     /gene="bgyg"
                     /note="rat Agc1 exon 1 sequence"
     intron          1619..2673
                     /gene="bgyg"
     CDS             2855..5896
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             5912..6946
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     promoter        complement(7937..7955)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7973..7989)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7997..8013
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8021..8051)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8066..8087)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8375..8963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9137..9994)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9995..10099)
                     /label=AmpR promoter
     rep_origin      10433..10888
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     11029..11045
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"