p4wtVT-lacI vector (V009717)

LOCUS       40924_2857        6644 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p4wtVT-lacI, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Boehm CR, Grant PK, Haseloff J.
  TITLE     Programmed emergence of a domain of gene expression in a bacterial 
            population
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Boehm CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2016) Plant Sciences, University of Cambridge, 
            Downing Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom
REFERENCE   3  (bases 1 to 6644)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6644)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-OCT-2016) Plant Sciences, University of Cambridge, Downing 
            Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             19..966
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     promoter        1179..1197
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1233..1244
                     /label=BBa_B0034
                     /note="BBa_B0034"
                     /regulatory_class="ribosome_binding_site"
     RBS             1233..1244
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1251..1967
                     /label=Venus
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
     terminator      1990..2061
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2077..2104
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     regulatory      2111..2145
                     /label=PJ23101*
                     /note="PJ23101*"
                     /regulatory_class="promoter"
     RBS             2154..2165
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2181..3260
                     /label=lacI
                     /note="lac repressor"
     CDS             3261..3293
                     /label=ssrA tag (LVA)
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
     terminator      complement(3344..3387)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3428..3455
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    3462..3482
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             3586..4443
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      4457..4516
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(4576..4605)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     misc_feature    4652..4673
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     terminator      complement(4680..4707)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(4723..4794)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(4817..5533)
                     /label=mTurquoise2
                     /note="enhanced monomeric variant of CFP (Goedhart et al., 
                     2012)"
     regulatory      complement(5534..5551)
                     /label=BBa_B0034
                     /note="BBa_B0034"
                     /regulatory_class="ribosome_binding_site"
     RBS             5540..5551
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      complement(5560..5594)
                     /label=pJ23101
                     /note="pJ23101"
                     /regulatory_class="promoter"
     misc_feature    5595..5615
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      5651..5682
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     terminator      5807..5901
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      6393..6615
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"