p4eng3VT-lacI vector (V009722)

LOCUS       40924_2832        6671 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p4eng3VT-lacI, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Boehm CR, Grant PK, Haseloff J.
  TITLE     Programmed emergence of a domain of gene expression in a bacterial 
            population
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6671)
  AUTHORS   Boehm CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2016) Plant Sciences, University of Cambridge, 
            Downing Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom
REFERENCE   3  (bases 1 to 6671)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6671)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-OCT-2016) Plant Sciences, University of Cambridge, Downing 
            Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             19..966
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     RBS             1244..1266
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1278..1994
                     /label=Venus
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
     terminator      2017..2088
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2104..2131
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     regulatory      2138..2172
                     /label=PJ23101*
                     /note="PJ23101*"
                     /regulatory_class="promoter"
     RBS             2181..2192
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2208..3287
                     /label=lacI
                     /note="lac repressor"
     CDS             3288..3320
                     /label=ssrA tag (LVA)
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
     terminator      complement(3371..3414)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3455..3482
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    3489..3509
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             3613..4470
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      4484..4543
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(4603..4632)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     misc_feature    4679..4700
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     terminator      complement(4707..4734)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(4750..4821)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(4844..5560)
                     /label=mTurquoise2
                     /note="enhanced monomeric variant of CFP (Goedhart et al., 
                     2012)"
     RBS             5567..5578
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      complement(5587..5621)
                     /label=pJ23101
                     /note="pJ23101"
                     /regulatory_class="promoter"
     misc_feature    5622..5642
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      5678..5709
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     terminator      5834..5928
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      6420..6642
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"