p3FsHMGB1A-wt vector (V009737)

LOCUS       40924_2662        7527 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p3FsHMGB1A-wt DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Kikuchi H, Yagi H, Hasegawa H, Ishii Y, Okabayashi K, Tsuruta M, 
            Hoshino G, Takayanagi A, Kitagawa Y.
  TITLE     Therapeutic potential of transgenic mesenchymal stem cells 
            engineered to mediate anti-high mobility group box 1 activity: 
            targeting of colon cancer
  JOURNAL   J. Surg. Res. 190 (1), 134-143 (2014)
  PUBMED    24679698
REFERENCE   2  (bases 1 to 7527)
  AUTHORS   Takayanagi A, Yagi H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-2014) Contact:Atsushi Takayanagi Keio University, 
            School of Medicine, Molecular Biology; Shinanomachi 35, Shinjuku, 
            Tokyo 160-8582, Japan
REFERENCE   3  (bases 1 to 7527)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7527)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Surg. 
            Res."; date: "2014"; volume: "190"; issue: "1"; pages: "134-143"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JAN-2014) Contact:Atsushi Takayanagi Keio University, School of 
            Medicine, Molecular Biology; Shinanomachi 35, Shinjuku, Tokyo 
            160-8582, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(66..654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    762..810
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    821..912
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    927..998
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     misc_feature    1034..1105
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     enhancer        1113..1491
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1524..1735
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1752..2020
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     intron          2044..2305
                     /note="bAct/bGlo hybrid Intron"
     sig_peptide     2334..2396
                     /note="enhanced human IgKappa secretion signal"
     CDS             2397..2462
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
     misc_feature    2792..3380
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    complement(3402..3523)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      3615..3641
                     /label=E.coli
                     /note="E.coli"
                     /regulatory_class="terminator"
     misc_feature    complement(3747..3949)
                     /label=human TOPO1 poly(A) region
                     /note="human TOPO1 poly(A) region"
     CDS             complement(3956..4972)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     misc_feature    complement(4999..6541)
                     /label=Intron
                     /note="Intron"
     regulatory      complement(5037..5104)
                     /label=E.coli EM2KC promoter
                     /note="E.coli EM2KC promoter"
                     /regulatory_class="promoter"
     misc_feature    5222..6198
                     /label=human TOP1 MARII
                     /note="human TOP1 MARII"
     misc_feature    6044..6198
                     /label=putative topoII binding region
                     /note="putative topoII binding region"
     misc_feature    6224..6250
                     /label=synthetic SP1-binding site
                     /note="synthetic SP1-binding site"
     regulatory      complement(6542..7523)
                     /gene="hTOP1"
                     /note="del-ATG; human TOP1"
                     /regulatory_class="promoter"
     gene            complement(6542..7523)
                     /gene="hTOP1"
                     /label=hTOP1
     misc_feature    6624
                     /label=del-Esp3I
                     /note="del-Esp3I"