Basic Vector Information
- Vector Name:
- p3FsHMGB1A-wt
- Length:
- 7527 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Kikuchi H, Yagi H, Hasegawa H, Ishii Y, Okabayashi K, Tsuruta M, Hoshino G, Takayanagi A, Kitagawa Y.
- Promoter:
- EF-1α core
p3FsHMGB1A-wt vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
p3FsHMGB1A-wt vector Sequence
LOCUS 40924_2662 7527 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector p3FsHMGB1A-wt DNA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7527)
AUTHORS Kikuchi H, Yagi H, Hasegawa H, Ishii Y, Okabayashi K, Tsuruta M,
Hoshino G, Takayanagi A, Kitagawa Y.
TITLE Therapeutic potential of transgenic mesenchymal stem cells
engineered to mediate anti-high mobility group box 1 activity:
targeting of colon cancer
JOURNAL J. Surg. Res. 190 (1), 134-143 (2014)
PUBMED 24679698
REFERENCE 2 (bases 1 to 7527)
AUTHORS Takayanagi A, Yagi H.
TITLE Direct Submission
JOURNAL Submitted (14-JAN-2014) Contact:Atsushi Takayanagi Keio University,
School of Medicine, Molecular Biology; Shinanomachi 35, Shinjuku,
Tokyo 160-8582, Japan
REFERENCE 3 (bases 1 to 7527)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7527)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J. Surg.
Res."; date: "2014"; volume: "190"; issue: "1"; pages: "134-143"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(14-JAN-2014) Contact:Atsushi Takayanagi Keio University, School of
Medicine, Molecular Biology; Shinanomachi 35, Shinjuku, Tokyo
160-8582, Japan"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7527
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(66..654)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
polyA_signal 762..810
/label=poly(A) signal
/note="synthetic polyadenylation signal"
misc_feature 821..912
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
misc_feature 927..998
/label=5' beta-globin insulator
/note="insulator upstream of the human beta-globin locus
(Farrell et al., 2002)"
misc_feature 1034..1105
/label=3' beta-globin insulator
/note="insulator downstream of the human beta-globin locus
(Farrell et al., 2002)"
enhancer 1113..1491
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1524..1735
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 1752..2020
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
intron 2044..2305
/note="bAct/bGlo hybrid Intron"
sig_peptide 2334..2396
/note="enhanced human IgKappa secretion signal"
CDS 2397..2462
/label=3xFLAG
/note="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
misc_feature 2792..3380
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
polyA_signal complement(3402..3523)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
regulatory 3615..3641
/label=E.coli
/note="E.coli"
/regulatory_class="terminator"
misc_feature complement(3747..3949)
/label=human TOPO1 poly(A) region
/note="human TOPO1 poly(A) region"
CDS complement(3956..4972)
/label=HygR
/note="aminoglycoside phosphotransferase from E. coli"
misc_feature complement(4999..6541)
/label=Intron
/note="Intron"
regulatory complement(5037..5104)
/label=E.coli EM2KC promoter
/note="E.coli EM2KC promoter"
/regulatory_class="promoter"
misc_feature 5222..6198
/label=human TOP1 MARII
/note="human TOP1 MARII"
misc_feature 6044..6198
/label=putative topoII binding region
/note="putative topoII binding region"
misc_feature 6224..6250
/label=synthetic SP1-binding site
/note="synthetic SP1-binding site"
regulatory complement(6542..7523)
/gene="hTOP1"
/note="del-ATG; human TOP1"
/regulatory_class="promoter"
gene complement(6542..7523)
/gene="hTOP1"
/label=hTOP1
misc_feature 6624
/label=del-Esp3I
/note="del-Esp3I"
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