Basic Vector Information
p35S-Zeo vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
p35S-Zeo vector Sequence
LOCUS 40924_2637 4902 bp DNA circular SYN 18-DEC-2018 DEFINITION Moss transformation vector p35S-Zeo, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4902) AUTHORS Hiwatashi Y, Hasebe M. TITLE Construction of knockout vectors for Physcomitrella patens JOURNAL Unpublished REFERENCE 2 (bases 1 to 4902) AUTHORS Hiwatashi Y, Hasebe M. TITLE Direct Submission JOURNAL Submitted (23-FEB-2007) Evolutionary Biology, National Institute for Basic Biology, Nishigounaka 38, Myoudaiji, Okazaki, Aichi 444-8585, Japan REFERENCE 3 (bases 1 to 4902) TITLE Direct Submission REFERENCE 4 (bases 1 to 4902) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (23-FEB-2007) Evolutionary Biology, National Institute for Basic Biology, Nishigounaka 38, Myoudaiji, Okazaki, Aichi 444-8585, Japan" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4902 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 24..128 /label=AmpR promoter CDS 129..986 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1160..1748 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2036..2057 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 2072..2102 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2110..2126 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2134..2150 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 2171..2189 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind 2219..2235 /label=KS primer /note="common sequencing primer, one of multiple similar variants" promoter 2729..3071 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" CDS 3078..3449 /codon_start=1 /label=BleoR /note="antibiotic-binding protein" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVLVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 3522..3698 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" primer_bind complement(4210..4226) /label=SK primer /note="common sequencing primer, one of multiple similar variants" promoter complement(4259..4277) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4287..4303) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 4445..4900 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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