p35S-BSD2 vector (V009751)

Basic Vector Information

      • Vector Name:
      • p35S-BSD2
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 4635 bp
      • Type:
      • Moss transformation vector
      • Source/Author:
      • Koshimizu S, Aono N, Sasaki-Sekimoto Y, Shigenobu S, Shimojima M, Ohta H, Kofuji R, Tamada Y, Murata T, Hasebe M.

p35S-BSD2 vector Vector Map

p35S-BSD24635 bp600120018002400300036004200f1 oriM13 fwdT7 promoterKS primerCaMV 35S promoterBSDCaMV poly(A) signalSK primerT3 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

p35S-BSD2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_2617        4635 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Moss transformation vector p35S-BSD2 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Koshimizu S, Aono N, Sasaki-Sekimoto Y, Shigenobu S, Shimojima M, 
            Ohta H, Kofuji R, Tamada Y, Murata T, Hasebe M.
  TITLE     Physcomitrella MADS-box genes regulate external water conduction and
            sperm movement necessary for fertilization
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Hiwatashi Y, Koshimizu S, Hasebe M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2017) Contact:Shizuka Koshimizu National Institute
            for Basic Biology, Division of Evolutionary Biology; Nishigonaka 38,
            Myodaiji, Okazaki, Aichi 444-8585, Japan URL 
            :http://www.nibb.ac.jp/evodevo/
REFERENCE   3  (bases 1 to 4635)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4635)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JUN-2017) Contact:Shizuka Koshimizu National Institute for Basic
            Biology, Division of Evolutionary Biology; Nishigonaka 38, Myodaiji,
            Okazaki, Aichi 444-8585, Japan URL :http://www.nibb.ac.jp/evodevo/"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        766..1111
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1150..1536
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="PLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGVNV
                     YHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIKAI
                     VKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    1682..1858
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(2394..2410)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2447..2465)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2486..2502)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2510..2526)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2534..2564)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2579..2600)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2888..3476)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3650..4507)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4508..4612)
                     /label=AmpR promoter

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