p34E-TpTer vector (V009761)

MSDS

Basic Vector Information

- - Vector Name:
  - p34E-TpTer
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 3808 bp
- - Type:
  - Cloning vector
- - Replication origin:
  - ori
- - Source/Author:
  - Shastri S, Spiewak HL, Sofoluwe A, Eidsvaag VA, Asghar AH, Pereira T, Bull EH, Butt AT, Thomas MS.
- - Promoter:
  - Pc

p34E-TpTer vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

p34E-TpTer vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_2567        3808 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p34E-TpTer, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Shastri S, Spiewak HL, Sofoluwe A, Eidsvaag VA, Asghar AH, Pereira 
            T, Bull EH, Butt AT, Thomas MS.
  TITLE     An efficient system for the generation of marked genetic mutants in 
            members of the genus Burkholderia
  JOURNAL   Plasmid (2016) In press
  PUBMED    27825973
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Shastri S, Spiewak HL, Pereira T, Sofoluwe AO, Eidsvaag VA, Bull EH,
            Asghar AH, Thomas MS.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 3808)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3808)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid 
            (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JUN-2016) Department of Infection, Immunity "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        786..890
                     /label=AmpR promoter
     CDS             891..1748
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1922..2510
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2762..2780
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(2789..2845)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      complement(2890..2917)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(3009..3095)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(3122..3355)
                     /codon_start=1
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     promoter        complement(3681..3709)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    3729..3785
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(3790..3808)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"

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