Basic Vector Information
p34E-Tp vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
p34E-Tp vector Sequence
LOCUS 40924_2562 3526 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector p34E-Tp, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3526) AUTHORS Shastri S, Spiewak HL, Sofoluwe A, Eidsvaag VA, Asghar AH, Pereira T, Bull EH, Butt AT, Thomas MS. TITLE An efficient system for the generation of marked genetic mutants in members of the genus Burkholderia JOURNAL Plasmid (2016) In press PUBMED 27825973 REFERENCE 2 (bases 1 to 3526) AUTHORS Shastri S, Spiewak HL, Pereira T, Sofoluwe AO, Eidsvaag VA, Bull EH, Asghar AH, Thomas MS. TITLE Direct Submission JOURNAL REFERENCE 3 (bases 1 to 3526) TITLE Direct Submission REFERENCE 4 (bases 1 to 3526) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid (2016) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (30-JUN-2016) Department of Infection, Immunity " COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..3526 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 786..890 /label=AmpR promoter CDS 891..1748 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1922..2510 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 2762..2780 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature complement(2789..2845) /label=MCS /note="pUC18/19 multiple cloning site" promoter 2856..2884 /label=Pc promoter /note="class 1 integron promoter" CDS 3210..3443 /codon_start=1 /label=TpR /note="E. coli plasmid-associated dihydrofolate reductase" /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW YCTKLTPEGYAVESESHPGSVQIYPVAALERVA" misc_feature 3447..3503 /label=MCS /note="pUC18/19 multiple cloning site" promoter complement(3508..3526) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase"
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