p34E-Cm2 vector (V009764)

LOCUS       V009764                 3736 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009764
VERSION     V009764
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Shastri S, Spiewak HL, Sofoluwe A, Eidsvaag VA, Asghar AH, Pereira
            T, Bull EH, Butt AT, Thomas MS.
  TITLE     An efficient system for the generation of marked genetic mutants in
            members of the genus Burkholderia
  JOURNAL   Plasmid (2016) In press
   PUBMED   27825973
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Shastri S, Spiewak HL, Pereira T, Sofoluwe AO, Eidsvaag VA, Bull EH,
            Asghar AH, Thomas MS.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3736)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3736)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid
            (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JUN-2016) Department of Infection, Immunity "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        786..890
                     /label="AmpR promoter"
     CDS             891..1748
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1922..2510
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        2762..2780
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(2789..2845)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(2884..3522)
                     /gene="cmlA"
                     /label="Chloramphenicol acetyltransferase 2"
                     /note="Chloramphenicol acetyltransferase 2 from Escherichia
                     coli. Accession#: P22615"
     regulatory      complement(3628..3656)
                     /label="catA2 promoter"
                     /note="catA2 promoter"
                     /regulatory_class="promoter"
     misc_feature    3657..3713
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(3718..3736)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"