Basic Vector Information
p2CABA vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
p2CABA vector Sequence
LOCUS 40924_2513 13038 bp DNA circular SYN 18-DEC-2018
DEFINITION Bi-directional promoter binary vector p2CABA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13038)
AUTHORS Tindamanyire JM, Townsley B, Kiggundu A, Sinha N, Tushemereirwe W.
TITLE Building a bi-directional promoter binary vector from the intergenic
region of Arabidopsis thaliana cab1 and cab2 divergent genes
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 13038)
AUTHORS Tindamanyire JM, Townsley B, Kiggundu A, Sinha N, Tushemereirwe W.
TITLE Direct Submission
JOURNAL Submitted (20-APR-2012) National Banana Research Programme, National
Agricultural Research Laboratories, 13 Kms Kampala-Gulu Highway,
Kampala, Wakiso 256, Uganda
REFERENCE 3 (bases 1 to 13038)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 13038)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(20-APR-2012) National Banana Research Programme, National
Agricultural Research Laboratories, 13 Kms Kampala-Gulu Highway,
Kampala, Wakiso 256, Uganda"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..13038
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator complement(1..707)
/label=OCS terminator
/note="octopine synthase terminator"
regulatory 735..2926
/note="Atca/b; from Arabidopsis thaliana chlorophyll a/b
gene; single natural bidirectional promoter that can drive
and express two different genes at the same time"
/regulatory_class="promoter"
5'UTR 735..800
/label=from chlorophyll a/b1 (CAB1) gene
/note="from chlorophyll a/b1 (CAB1) gene"
regulatory 2965..2974
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
protein_bind 2987..3111
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter 3136..3166
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
CDS 3220..3876
/label=CmR
/note="chloramphenicol acetyltransferase"
CDS 4221..4523
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(4567..4691)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
regulatory 4704..4914
/note="t35S; cauliflower mosaic virus 35S terminator"
/regulatory_class="terminator"
misc_feature 4977..5001
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 6301..6927
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS 7364..8428
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 8497..8691
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 9035..9175
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(9361..9949)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(10196..10984)
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
misc_feature 11512..11536
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
terminator complement(11629..11881)
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(11924..12715)
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
promoter complement(12749..12928)
/label=NOS promoter
/note="nopaline synthase promoter"
This page is informational only.