p2CABA vector (V009768)

LOCUS       40924_2513       13038 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Bi-directional promoter binary vector p2CABA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13038)
  AUTHORS   Tindamanyire JM, Townsley B, Kiggundu A, Sinha N, Tushemereirwe W.
  TITLE     Building a bi-directional promoter binary vector from the intergenic
            region of Arabidopsis thaliana cab1 and cab2 divergent genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13038)
  AUTHORS   Tindamanyire JM, Townsley B, Kiggundu A, Sinha N, Tushemereirwe W.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2012) National Banana Research Programme, National
            Agricultural Research Laboratories, 13 Kms Kampala-Gulu Highway, 
            Kampala, Wakiso 256, Uganda
REFERENCE   3  (bases 1 to 13038)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13038)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-APR-2012) National Banana Research Programme, National 
            Agricultural Research Laboratories, 13 Kms Kampala-Gulu Highway, 
            Kampala, Wakiso 256, Uganda"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..707)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     regulatory      735..2926
                     /note="Atca/b; from Arabidopsis thaliana chlorophyll a/b
                     gene; single natural bidirectional promoter that can drive 
                     and express two different genes at the same time"
                     /regulatory_class="promoter"
     5'UTR           735..800
                     /label=from chlorophyll a/b1 (CAB1) gene
                     /note="from chlorophyll a/b1 (CAB1) gene"
     regulatory      2965..2974
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    2987..3111
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3136..3166
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             3220..3876
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             4221..4523
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(4567..4691)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      4704..4914
                     /note="t35S; cauliflower mosaic virus 35S terminator"
                     /regulatory_class="terminator"
     misc_feature    4977..5001
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6301..6927
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             7364..8428
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      8497..8691
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9035..9175
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9361..9949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10196..10984)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     misc_feature    11512..11536
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(11629..11881)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11924..12715)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(12749..12928)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"