Basic Vector Information
p2059-URA-RS vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
p2059-URA-RS vector Sequence
LOCUS 40924_2483 3230 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector p2059-URA-RS, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3230) AUTHORS Pachlinger R, Mitterbauer R, Adam G, Strauss J. TITLE Metabolically independent and accurately adjustable Aspergillus sp. expression system JOURNAL Appl. Environ. Microbiol. 71 (2), 672-678 (2005) PUBMED 15691916 REFERENCE 2 (bases 1 to 3230) AUTHORS Pachlinger R, Mitterbauer R, Adam G, Strauss J. TITLE Direct Submission JOURNAL Submitted (24-JUN-2004) Department of Applied Plant Sciences and Plant Biotechnology, BOKU - University of Natural Resources and Applied Life Sciences, Vienna, Muthgasse 18, Vienna A-1180, Austria REFERENCE 3 (bases 1 to 3230) TITLE Direct Submission REFERENCE 4 (bases 1 to 3230) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Appl. Environ. Microbiol."; date: "2005"; volume: "71"; issue: "2"; pages: "672-678" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (24-JUN-2004) Department of Applied Plant Sciences and Plant Biotechnology, BOKU - University of Natural Resources and Applied Life Sciences, Vienna, Muthgasse 18, Vienna A-1180, Austria" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..3230 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin complement(6..461) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 603..619 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 626..644 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 677..693 /label=SK primer /note="common sequencing primer, one of multiple similar variants" misc_feature complement(697..785) /label=random sequence (RS) fragment derived from ampR /note="random sequence (RS) fragment derived from ampR" misc_feature complement(791..907) /label=Saccharomyces cerevisiae URA3 promoter fragment /note="Saccharomyces cerevisiae URA3 promoter fragment" misc_feature complement(908..946) /label=3xERE (estrogen responsive element) /note="3xERE (estrogen responsive element)" primer_bind complement(999..1015) /label=KS primer /note="common sequencing primer, one of multiple similar variants" promoter complement(1045..1063) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(1084..1100) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(1108..1124) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1132..1162) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(1177..1198) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(1486..2074) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2248..3105) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3106..3210) /label=AmpR promoter
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