p2059-URA-RS vector (V009772)

Basic Vector Information

      • Vector Name:
      • p2059-URA-RS
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 3230 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Pachlinger R, Mitterbauer R, Adam G, Strauss J.

p2059-URA-RS vector Vector Map

p2059-URA-RS3230 bp6001200180024003000f1 oriM13 fwdT7 promoterSK primerrandom sequence (RS) fragment derived from ampRSaccharomyces cerevisiae URA3 promoter fragment3xERE (estrogen responsive element)KS primerT3 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

p2059-URA-RS vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_2483        3230 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p2059-URA-RS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Pachlinger R, Mitterbauer R, Adam G, Strauss J.
  TITLE     Metabolically independent and accurately adjustable Aspergillus sp. 
            expression system
  JOURNAL   Appl. Environ. Microbiol. 71 (2), 672-678 (2005)
  PUBMED    15691916
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Pachlinger R, Mitterbauer R, Adam G, Strauss J.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUN-2004) Department of Applied Plant Sciences and 
            Plant Biotechnology, BOKU - University of Natural Resources and 
            Applied Life Sciences, Vienna, Muthgasse 18, Vienna A-1180, Austria
REFERENCE   3  (bases 1 to 3230)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3230)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2005"; volume: "71"; issue: "2"; pages:
            "672-678"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-JUN-2004) Department of Applied Plant Sciences and Plant 
            Biotechnology, BOKU - University of Natural Resources and Applied 
            Life Sciences, Vienna, Muthgasse 18, Vienna A-1180, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     677..693
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(697..785)
                     /label=random sequence (RS) fragment derived from ampR
                     /note="random sequence (RS) fragment derived from ampR"
     misc_feature    complement(791..907)
                     /label=Saccharomyces cerevisiae URA3 promoter fragment
                     /note="Saccharomyces cerevisiae URA3 promoter fragment"
     misc_feature    complement(908..946)
                     /label=3xERE (estrogen responsive element)
                     /note="3xERE (estrogen responsive element)"
     primer_bind     complement(999..1015)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1045..1063)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1084..1100)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1108..1124)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1132..1162)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1177..1198)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1486..2074)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2248..3105)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3106..3210)
                     /label=AmpR promoter

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