p1168hIL6mETS-luc+ vector (V009790)

Basic Vector Information

      • Vector Name:
      • p1168hIL6mETS-luc+
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 5990 bp
      • Type:
      • Reporter vector
      • Source/Author:
      • De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.

p1168hIL6mETS-luc+ vector Vector Map

p1168hIL6mETS-luc+5990 bp600120018002400300036004200480054005' FR of hIL6hIL6 promoter mutantluciferaseSV40 poly(A) signaloriAmpRAmpR promoterf1 oripoly(A) signalpause site

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

p1168hIL6mETS-luc+ vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_2393        5990 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector p1168hIL6mETS-luc+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5990)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5990)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5990)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    32..672
                     /label=5' FR of hIL6
                     /note="5' FR of hIL6"
     regulatory      673..1208
                     /label=hIL6 promoter mutant
                     /note="hIL6 promoter mutant"
                     /regulatory_class="promoter"
     CDS             1256..2905
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(2949..3070)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3493..4081)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4255..5112)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5113..5217)
                     /label=AmpR promoter
     rep_origin      5244..5699
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5830..5878
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5892..5983
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"

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