Basic Vector Information
p-4725sox10.cre vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
p-4725sox10.cre vector Sequence
LOCUS 40924_2323 11499 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector p-4725sox10.cre, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11499)
AUTHORS Rodrigues FSL.M., Doughton G, Kelsh RN.
TITLE A novel transgenic line using the Cre-lox system to allow permanent
lineage labelling of the zebrafish neural crest
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 11499)
AUTHORS Rodrigues FSL.M., Kelsh RN.
TITLE Direct Submission
JOURNAL Submitted (16-FEB-2012) Biology and Biochemistry, University of
Bath, Claverton Down, Bath, Somerset BA2 7AY, UK
REFERENCE 3 (bases 1 to 11499)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 11499)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(16-FEB-2012) Biology and Biochemistry, University of Bath,
Claverton Down, Bath, Somerset BA2 7AY, UK"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..11499
/mol_type="other DNA"
/organism="synthetic DNA construct"
gene 27..7097
/gene="sox10"
/label=sox10
/note="derived from Danio rerio"
regulatory 27..4624
/gene="sox10"
/regulatory_class="promoter"
5'UTR join(4625..4756,6862..7097)
/gene="sox10"
exon 4625..4756
/gene="sox10"
/number=1
intron 4757..6861
/gene="sox10"
/number=1
exon 6862..7097
/gene="sox10"
/number=2
CDS 7133..8161
/label=Cre
/note="site-specific recombinase"
polyA_signal 8645..8693
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
primer_bind complement(8719..8735)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(8782..8800)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(8821..8837)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 8845..8861
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8869..8899)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(8914..8935)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(9223..9811)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9985..10842)
/label=AmpR
/note="beta-lactamase"
promoter complement(10843..10947)
/label=AmpR promoter
rep_origin complement(10973..11428)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
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