p-4725sox10.cre vector (V009812)

LOCUS       40924_2323       11499 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p-4725sox10.cre, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11499)
  AUTHORS   Rodrigues FSL.M., Doughton G, Kelsh RN.
  TITLE     A novel transgenic line using the Cre-lox system to allow permanent 
            lineage labelling of the zebrafish neural crest
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11499)
  AUTHORS   Rodrigues FSL.M., Kelsh RN.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2012) Biology and Biochemistry, University of 
            Bath, Claverton Down, Bath, Somerset BA2 7AY, UK
REFERENCE   3  (bases 1 to 11499)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11499)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-FEB-2012) Biology and Biochemistry, University of Bath, 
            Claverton Down, Bath, Somerset BA2 7AY, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            27..7097
                     /gene="sox10"
                     /label=sox10
                     /note="derived from Danio rerio"
     regulatory      27..4624
                     /gene="sox10"
                     /regulatory_class="promoter"
     5'UTR           join(4625..4756,6862..7097)
                     /gene="sox10"
     exon            4625..4756
                     /gene="sox10"
                     /number=1
     intron          4757..6861
                     /gene="sox10"
                     /number=1
     exon            6862..7097
                     /gene="sox10"
                     /number=2
     CDS             7133..8161
                     /label=Cre
                     /note="site-specific recombinase"
     polyA_signal    8645..8693
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(8719..8735)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(8782..8800)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8821..8837)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8845..8861
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8869..8899)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8914..8935)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9223..9811)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9985..10842)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10843..10947)
                     /label=AmpR promoter
     rep_origin      complement(10973..11428)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"