p(T5CkB)3-50huIL6P-luc+ vector (V009813)

MSDS

Basic Vector Information

Vector Name:: p(T5CkB)3-50huIL6P-luc+

Antibiotic Resistance:: Ampicillin

Length:: 4971 bp

Type:: Reporter vector

Replication origin:: ori

Source/Author:: De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.

p(T5CkB)3-50huIL6P-luc+ vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

p(T5CkB)3-50huIL6P-luc+ vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_2318        4971 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector p(T5CkB)3-50huIL6P-luc+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4971)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4971)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      78..95
                     /label=T5CkB
                     /note="T5CkB"
                     /regulatory_class="promoter"
     regulatory      96..113
                     /label=T5CkB
                     /note="T5CkB"
                     /regulatory_class="promoter"
     regulatory      114..131
                     /label=T5CkB
                     /note="T5CkB"
                     /regulatory_class="promoter"
     regulatory      144..193
                     /label=hIL6f
                     /note="hIL6f"
                     /regulatory_class="promoter"
     CDS             241..1890
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(1934..2055)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2474..3062)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3236..4093)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4094..4198)
                     /label=AmpR promoter
     rep_origin      4225..4680
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4811..4859
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4873..4964
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"

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