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pRSET-EGFP vector (V009825)

Basic Vector Information

Vector Name:
pRSET-EGFP
Antibiotic Resistance:
Ampicillin
Length:
3571 bp
Type:
Prokaryotic Expression Vectors
Replication origin:
ori
Selection Marker:
EGFP
Promoter:
T7

pRSET-EGFP vector Vector Map

pRSET-EGFP3571 bp6001200180024003000T7 promoterRBS6xHisT7 tag (gene 10 leader)Xpress(TM) tagEGFPT7 terminatorf1 oriAmpR promoterAmpRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pRSET-EGFP vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_38188        3571 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3571)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             70..92
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             112..129
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             133..165
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=leader peptide from bacteriophage T7 gene 10
                     /note="T7 tag (gene 10 leader)"
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             169..192
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     CDS             199..915
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      986..1033
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1130..1585
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1611..1715
                     /label=AmpR promoter
     CDS             1716..2573
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2747..3335
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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