Basic Vector Information
nvYFP w- vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
nvYFP w- vector Sequence
LOCUS 40924_2259 7641 bp DNA circular SYN 18-DEC-2018 DEFINITION Transformation vector nvYFP w-, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7641) AUTHORS Le T, Liang Z, Patel H, Yu MH, Sivasubramaniam G, Slovitt M, Tanentzapf G, Mohanty N, Paul SM, Wu VM, Beitel GJ. TITLE A new family of Drosophila balancer chromosomes with a w- dfd-GMR yellow fluorescent protein marker JOURNAL Genetics 174 (4), 2255-2257 (2006) PUBMED 17057238 REFERENCE 2 (bases 1 to 7641) AUTHORS Le T, Liang Z, Beitel GJ. TITLE Direct Submission JOURNAL Submitted (30-JUN-2006) Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA REFERENCE 3 (bases 1 to 7641) TITLE Direct Submission REFERENCE 4 (bases 1 to 7641) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; date: "2006"; volume: "174"; issue: "4"; pages: "2255-2257" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (30-JUN-2006) Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..7641 /mol_type="other DNA" /organism="synthetic DNA construct" regulatory 79..2813 /label=dfd HZ2.7 promoter /note="dfd HZ2.7 promoter" /regulatory_class="promoter" regulatory 2819..2999 /label=GMR enhancer /note="GMR enhancer" /regulatory_class="enhancer" CDS 3204..3920 /label=mVenus /note="Venus YFP with monomerizing A206K mutation (Nagai et al., 2002; Kremers et al., 2006)" polyA_signal 4196..4317 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(4882..5470) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5644..6501) /label=AmpR /note="beta-lactamase" promoter complement(6502..6606) /label=AmpR promoter misc_feature complement(7409..7641) /label=P element 3' end /note="P element 3' end"
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