nvYFP w- vector (V009829)

Basic Vector Information

      • Vector Name:
      • nvYFP w-
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 7641 bp
      • Type:
      • Transformation vector
      • Source/Author:
      • Le T, Liang Z, Patel H, Yu MH, Sivasubramaniam G, Slovitt M, Tanentzapf G, Mohanty N, Paul SM, Wu VM, Beitel GJ.

nvYFP w- vector Vector Map

nvYFP w-7641 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600690072007500dfd HZ2.7 promoterGMR enhancermVenusSV40 poly(A) signaloriAmpRAmpR promoterP element 3' end

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

nvYFP w- vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_2259        7641 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector nvYFP w-, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Le T, Liang Z, Patel H, Yu MH, Sivasubramaniam G, Slovitt M, 
            Tanentzapf G, Mohanty N, Paul SM, Wu VM, Beitel GJ.
  TITLE     A new family of Drosophila balancer chromosomes with a w- dfd-GMR 
            yellow fluorescent protein marker
  JOURNAL   Genetics 174 (4), 2255-2257 (2006)
  PUBMED    17057238
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Le T, Liang Z, Beitel GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2006) Biochemistry, Molecular Biology and Cell 
            Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 
            60208, USA
REFERENCE   3  (bases 1 to 7641)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7641)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "2006"; volume: "174"; issue: "4"; pages: "2255-2257"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JUN-2006) Biochemistry, Molecular Biology and Cell Biology, 
            Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      79..2813
                     /label=dfd HZ2.7 promoter
                     /note="dfd HZ2.7 promoter"
                     /regulatory_class="promoter"
     regulatory      2819..2999
                     /label=GMR enhancer
                     /note="GMR enhancer"
                     /regulatory_class="enhancer"
     CDS             3204..3920
                     /label=mVenus
                     /note="Venus YFP with monomerizing A206K mutation (Nagai et
                     al., 2002; Kremers et al., 2006)"
     polyA_signal    4196..4317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4882..5470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5644..6501)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6502..6606)
                     /label=AmpR promoter
     misc_feature    complement(7409..7641)
                     /label=P element 3' end
                     /note="P element 3' end"

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