Basic Vector Information
NOR gate BCD-RFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
NOR gate BCD-RFP vector Sequence
LOCUS 40924_2254 5009 bp DNA circular SYN 18-DEC-2018
DEFINITION Boolean integrase logic vector NOR gate BCD-RFP, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5009)
AUTHORS Courbet A, Endy D, Renard E, Molina F, Bonnet J.
TITLE Detection of pathological biomarkers in human clinical samples via
amplifying genetic switches and logic gates
JOURNAL Sci Transl Med 7 (289), 289RA83 (2015)
PUBMED 26019219
REFERENCE 2 (bases 1 to 5009)
AUTHORS Courbet A, Molina F, Endy D, Bonnet J.
TITLE Direct Submission
JOURNAL Submitted (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la
Valsiere, Montpellier, Herault 34184, France
REFERENCE 3 (bases 1 to 5009)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5009)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl
Med"; date: "2015"; volume: "7"; issue: "289"; pages: "289RA83"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la Valsiere,
Montpellier, Herault 34184, France"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..5009
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..22
/label=BioBrick prefix
/note="BioBrick prefix for parts that do not start with
'ATG'"
regulatory 23..69
/label=P7
/note="P7"
/regulatory_class="promoter"
misc_feature 70..122
/label=attB TP901-1
/note="attB TP901-1"
terminator complement(129..156)
/label=T7Te terminator
/note="phage T7 early transcription terminator"
terminator complement(172..243)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
misc_feature 252..301
/label=attP TP901-1
/note="attP TP901-1"
misc_feature 302..351
/label=attB Bxb1
/note="attB Bxb1"
regulatory 352..464
/label=J61048
/note="J61048"
/regulatory_class="terminator"
misc_feature 465..517
/label=attP Bxb1
/note="attP Bxb1"
misc_RNA 519..569
/label=sTRSV HHRz
/note="hammerhead ribozyme from the tobacco ringspot virus
satellite RNA (Khvorova et al., 2003)"
misc_feature 593..677
/label=BCD2
/note="BCD2"
RBS 663..671
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 675..1370
/label=mKate2
/note="monomeric far-red fluorescent protein (Shcherbo et
al., 2009)"
terminator 1393..1464
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1480..1507
/label=T7Te terminator
/note="phage T7 early transcription terminator"
misc_feature 1514..1534
/label=BioBrick suffix
/note="universal suffix for all parts"
terminator 1570..1601
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
terminator 1726..1820
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
rep_origin 2312..2534
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
CDS 2582..3529
/label=Rep101
/note="RepA protein needed for replication with the pSC101
origin"
CDS 3821..4678
/label=AmpR
/note="beta-lactamase"
terminator 4692..4751
/label=his operon terminator
/note="This putative transcriptin terminator from the E.
coli his operon has a 2-bp deletion introduced during
synthesis. Its efficiency has not been determined."
terminator 4811..4840
/label=T3Te terminator
/note="phage T3 early transcription terminator"
terminator complement(4934..4963)
/label=T3Te terminator
/note="phage T3 early transcription terminator"
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