NOR gate BCD-RFP vector (V009830)

LOCUS       40924_2254        5009 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Boolean integrase logic vector NOR gate BCD-RFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Courbet A, Endy D, Renard E, Molina F, Bonnet J.
  TITLE     Detection of pathological biomarkers in human clinical samples via 
            amplifying genetic switches and logic gates
  JOURNAL   Sci Transl Med 7 (289), 289RA83 (2015)
  PUBMED    26019219
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Courbet A, Molina F, Endy D, Bonnet J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la 
            Valsiere, Montpellier, Herault 34184, France
REFERENCE   3  (bases 1 to 5009)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5009)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med"; date: "2015"; volume: "7"; issue: "289"; pages: "289RA83"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la Valsiere, 
            Montpellier, Herault 34184, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      23..69
                     /label=P7
                     /note="P7"
                     /regulatory_class="promoter"
     misc_feature    70..122
                     /label=attB TP901-1
                     /note="attB TP901-1"
     terminator      complement(129..156)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(172..243)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    252..301
                     /label=attP TP901-1
                     /note="attP TP901-1"
     misc_feature    302..351
                     /label=attB Bxb1
                     /note="attB Bxb1"
     regulatory      352..464
                     /label=J61048
                     /note="J61048"
                     /regulatory_class="terminator"
     misc_feature    465..517
                     /label=attP Bxb1
                     /note="attP Bxb1"
     misc_RNA        519..569
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     misc_feature    593..677
                     /label=BCD2
                     /note="BCD2"
     RBS             663..671
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             675..1370
                     /label=mKate2
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     terminator      1393..1464
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1480..1507
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    1514..1534
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1570..1601
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     terminator      1726..1820
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2312..2534
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2582..3529
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     CDS             3821..4678
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      4692..4751
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      4811..4840
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      complement(4934..4963)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"