Basic Vector Information
- Vector Name:
- nLucFlucGW
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9789 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Reis RS.
- Promoter:
- lac UV5
nLucFlucGW vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
nLucFlucGW vector Sequence
LOCUS 40924_2239 9789 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector nLucFlucGW, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9789)
AUTHORS Reis RS.
TITLE nLuc/Fluc dual luciferase for protoplast transformation
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9789)
AUTHORS Reis RS.
TITLE Direct Submission
JOURNAL Submitted (29-JUN-2018) Department of Plant Molecular Biology,
University of Lausanne, Quartier UNIL-Sorge, Batiment Biophore,
Lausanne, Vaud 1015, Switzerland
REFERENCE 3 (bases 1 to 9789)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9789)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(29-JUN-2018) Department of Plant Molecular Biology, University of
Lausanne, Quartier UNIL-Sorge, Batiment Biophore, Lausanne, Vaud
1015, Switzerland"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..9789
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator 103..189
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 281..308
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
regulatory 324..1449
/label=RHIP1pro
/note="RHIP1pro"
/regulatory_class="promoter"
protein_bind 1470..1594
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter 1619..1649
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
CDS 1703..2359
/label=CmR
/note="chloramphenicol acetyltransferase"
CDS 2704..3006
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(3050..3174)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
CDS 3188..3253
/label=F2A
/note="2A peptide from foot-and-mouth disease virus
polyprotein"
CDS 3272..3784
/label=Nluc
/note="NanoLuc(R) luciferase"
CDS 3785..3811
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
terminator 3834..4082
/label=HSP terminator
/note="efficient transcription terminator from the
Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya
et al., 2010)"
protein_bind complement(4091..4115)
/label=attB2
/note="recombination site for the Gateway(R) BP reaction"
CDS 5850..7499
/label=luciferase
/note="firefly luciferase"
terminator 7529..7781
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
promoter 8118..8188
/label=AmpR promoter
CDS 8189..9046
/label=AmpR
/note="beta-lactamase"
rep_origin 9139..9727
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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