Basic Vector Information
- Vector Name:
- nanos-Cre vetor
- Antibiotic Resistance:
- Ampicillin
- Length:
- 12006 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Laktionov P, Maksimov D, White-Cooper H, Belyakin S.
nanos-Cre vetor vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
nanos-Cre vetor vector Sequence
LOCUS 40924_2184 12006 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector nanos-Cre vetor, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12006)
AUTHORS Laktionov P, Maksimov D, White-Cooper H, Belyakin S.
TITLE Cookie monster binding to the repressed chromosomal domains is
needed for selective activation of testis-specific genes in
Drosophila melanogaster
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 12006)
AUTHORS Laktionov P.
TITLE Direct Submission
JOURNAL Submitted (29-MAR-2013) Genomics lab, Institute of molecular and
cellular biology, Lavrentiev ave 8/2, Novosibirsk 630090, Russia
REFERENCE 3 (bases 1 to 12006)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 12006)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(29-MAR-2013) Genomics lab, Institute of molecular and cellular
biology, Lavrentiev ave 8/2, Novosibirsk 630090, Russia"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..12006
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 46..1022
/note="Drosophila melanogaster genomic DNA region upstream
the nanos gene"
CDS 1033..2061
/label=Cre
/note="site-specific recombinase"
misc_feature 2073..3202
/note="Drosophila melanogaster genomic DNA region
downstream the nanos gene"
intron 3286..3351
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 3481..3501
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 3773..3907
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
gene 4259..7826
/gene="mini-white"
/label=mini-white
protein_bind 8005..8074
/label=attB
/note="attB site for the phi-C31 integrase (Groth et al.,
2000)"
misc_feature complement(8428..9013)
/label=P element 5' end
rep_origin complement(9248..9836)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(10010..10867)
/label=AmpR
/note="beta-lactamase"
promoter complement(10868..10972)
/label=AmpR promoter
misc_feature complement(11774..12006)
/label=P element 3' end
/note="P element 3' end"
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