Basic Vector Information
nanos-Cre vetor vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
nanos-Cre vetor vector Sequence
LOCUS Exported 12006 bp ds-DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector nanos-Cre vetor, complete sequence.
ACCESSION KC845567
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12006)
AUTHORS Laktionov P, Maksimov D, White-Cooper H, Belyakin S.
TITLE Cookie monster binding to the repressed chromosomal domains is
needed for selective activation of testis-specific genes in
Drosophila melanogaster
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 12006)
AUTHORS Laktionov P.
TITLE Direct Submission
JOURNAL Submitted (29-MAR-2013) Genomics lab, Institute of molecular and
cellular biology, Lavrentiev ave 8/2, Novosibirsk 630090, Russia
REFERENCE 3 (bases 1 to 12006)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..12006
/organism="Cloning vector nanos-Cre vetor"
/mol_type="other DNA"
/db_xref="taxon:1366864"
misc_feature 46..1022
/note="Drosophila melanogaster genomic DNA region upstream
the nanos gene"
CDS 1033..2064
/codon_start=1
/gene="Cre"
/product="Cre recombinase"
/label=Cre
/protein_id="AGR55448.1"
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
gene 1033..2064
/gene="Cre"
/label=Cre
misc_feature 2073..3202
/note="Drosophila melanogaster genomic DNA region
downstream the nanos gene"
intron 3286..3351
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 3481..3501
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 3773..3907
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
gene 4259..7826
/gene="mini-white"
/label=mini-white
misc_recomb 7979..8302
/label=phiC31 attB site
/note="phiC31 attB site"
protein_bind 8005..8074
/label=attB
/bound_moiety="phage phi-C31 integrase"
/note="attB site for the phi-C31 integrase (Groth et al.,
2000)"
misc_feature 8428..9013
/label=P element 5' end
rep_origin complement(9248..9836)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(10007..10867)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=bla
/note="ampicillin resistance"
/protein_id="AGR55449.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
gene complement(10007..10867)
/gene="bla"
/label=bla
promoter complement(10868..10972)
/gene="bla"
/label=AmpR promoter
misc_feature 11774..12006
/label=P element 3' end
/note="P element 3' end"
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