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nanos-Cre vetor vector (V009837#)

Basic Vector Information

Basic Vector Information
Vector Name nanos-Cre vetor Antibiotic Resistance Ampicillin
Length 12006 bp Type Cloning vector
Source Laktionov P, Maksimov D, White-Cooper H, Belyakin S.

nanos-Cre vetor vector Vector Map

nanos-Cre vetor12006 bp60012001800240030003600420048005400600066007200780084009000960010200108001140012000Drosophila melanogaster genomic DNA region upstream the nanos geneCreDrosophila melanogaster genomic DNA region downstream the nanos genesmall t intronSV40 NLSSV40 poly(A) signalmini-whitephiC31 attB siteP element 5' endoriblaAmpR promoterP element 3' end

Plasmid Resuspension protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

nanos-Cre vetor vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported               12006 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector nanos-Cre vetor, complete sequence.
ACCESSION   KC845567
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12006)
  AUTHORS   Laktionov P, Maksimov D, White-Cooper H, Belyakin S.
  TITLE     Cookie monster binding to the repressed chromosomal domains is 
            needed for selective activation of testis-specific genes in 
            Drosophila melanogaster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12006)
  AUTHORS   Laktionov P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2013) Genomics lab, Institute of molecular and 
            cellular biology, Lavrentiev ave 8/2, Novosibirsk 630090, Russia
REFERENCE   3  (bases 1 to 12006)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12006
                     /organism="Cloning vector nanos-Cre vetor"
                     /mol_type="other DNA"
                     /db_xref="taxon:1366864"
     misc_feature    46..1022
                     /note="Drosophila melanogaster genomic DNA region upstream 
                     the nanos gene"
     CDS             1033..2064
                     /codon_start=1
                     /gene="Cre"
                     /product="Cre recombinase"
                     /label=Cre
                     /protein_id="AGR55448.1"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     gene            1033..2064
                     /gene="Cre"
                     /label=Cre
     misc_feature    2073..3202
                     /note="Drosophila melanogaster genomic DNA region 
                     downstream the nanos gene"
     intron          3286..3351
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3481..3501
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3773..3907
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            4259..7826
                     /gene="mini-white"
                     /label=mini-white
     misc_recomb     7979..8302
                     /label=phiC31 attB site
                     /note="phiC31 attB site"
     protein_bind    8005..8074
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    8428..9013
                     /label=P element 5' end
     rep_origin      complement(9248..9836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10007..10867)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance"
                     /protein_id="AGR55449.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(10007..10867)
                     /gene="bla"
                     /label=bla
     promoter        complement(10868..10972)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    11774..12006
                     /label=P element 3' end
                     /note="P element 3' end"

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