Basic Vector Information
| Basic Vector Information | |||
|---|---|---|---|
| Vector Name | mini-CTX-GFP | Antibiotic Resistance | Tetracycline |
| Length | 6370 bp | Type | Integration vector |
| Source | Hoang TT, Kutchma AJ, Becher A, Schweizer HP. | ||
mini-CTX-GFP vector Vector Map
Plasmid Resuspension protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
mini-CTX-GFP vector Sequence
LOCUS Exported 6370 bp ds-DNA circular SYN 17-DEC-2018
DEFINITION Integration vector mini-CTX-GFP, complete sequence.
ACCESSION AF140578
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6370)
AUTHORS Hoang TT, Kutchma AJ, Becher A, Schweizer HP.
TITLE A site-specific gene integration system for Pseudomonas aeruginosa
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6370)
AUTHORS Hoang TT, Kutchma AJ, Becher A, Schweizer HP.
TITLE Direct Submission
JOURNAL Submitted (06-APR-1999) Microbiology, Colorado State University,
Fort Collins, CO 80523, USA
REFERENCE 3 (bases 1 to 6370)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6370
/organism="Integration vector mini-CTX-GFP"
/lab_host="Pseudomonas aeruginosa"
/mol_type="other DNA"
/db_xref="taxon:106408"
protein_bind 238..285
/label=Flp recombinase binding site
/bound_moiety="Flp recombinase"
/note="Flp recombinase target site"
misc_feature 495..521
/label=phage CTX attachment site, core sequence
/note="phage CTX attachment site, core sequence"
regulatory 745..769
/regulatory_class="terminator"
/note="T4 transcription termination signal"
regulatory 827..846
/regulatory_class="promoter"
/note="T3 promoter"
promoter 827..845
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind 882..898
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(908..1624)
/codon_start=1
/gene="GFP"
/product="green fluorescent protein"
/label=GFP
/protein_id="AAF06117.1"
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
gene complement(908..1624)
/gene="GFP"
/label=GFP
/note="GFP mut2 variant"
primer_bind complement(1692..1708)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
regulatory 1733..1752
/regulatory_class="promoter"
/note="T7 promoter"
promoter complement(1734..1752)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 1811..1835
/regulatory_class="terminator"
/note="T4 transcription termination signal"
protein_bind 1926..1973
/label=Flp recombinase binding site
/bound_moiety="Flp recombinase"
/note="Flp recombinase target site"
oriT 2173..2432
oriT 2180..2288
/note="incP origin of transfer"
CDS complement(2447..3616)
/codon_start=1
/gene="int"
/product="integrase"
/label=int
/protein_id="AAF06118.1"
/translation="MADGVEVRGKRIRIYFRYQGELCRESIPGDATPENIANAERLAGI
INYEIKQGVFSYSRHFPDSPRVKSNTLGHYIDLWLDIKRNQIAASGFRGYTSRVETHIR
PRWGDSQADSIDHLDIQDWVQNTLMPKLHNKTVREIVSNLRQIFRLYRTRNRSAHDPTD
GIVITLPDADDPDPFTREEIDLILGTETARIGELNLAEFMIWSGPRVSEAIALAWEDVD
LDTGTVVFRRARVRSQYKVTKTRRSTRKVQLLAPALRALQQQAKLTRRLPPVQIEVIDR
DNRTRKPQRVRFVFHNSASGAAYSTSDTLRNGWWHGHLRNAGVRSRGPNQCRHTFASQM
LSSGIATPEWIADQMGHTSTAMIFKHYAKWISKDGPDIVGLLNQALKLS"
gene complement(2447..3616)
/gene="int"
/label=int
/note="modified phage CTX integrase gene"
rep_origin complement(3713..4301)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5123..6313)
/codon_start=1
/gene="tet"
/product="Tet protein"
/label=tet
/protein_id="AAF06119.1"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
gene complement(5123..6313)
/gene="tet"
/label=tet
/note="modified tet gene"
promoter complement(6361..19)
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
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