Basic Vector Information
- Vector Name:
- mini-CTX-GFP
- Antibiotic Resistance:
- Tetracycline
- Length:
- 6370 bp
- Type:
- Integration vector
- Replication origin:
- ori
- Source/Author:
- Hoang TT, Kutchma AJ, Becher A, Schweizer HP.
mini-CTX-GFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
mini-CTX-GFP vector Sequence
LOCUS 40924_1964 6370 bp DNA circular SYN 17-DEC-2018
DEFINITION Integration vector mini-CTX-GFP, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6370)
AUTHORS Hoang TT, Kutchma AJ, Becher A, Schweizer HP.
TITLE A site-specific gene integration system for Pseudomonas aeruginosa
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6370)
AUTHORS Hoang TT, Kutchma AJ, Becher A, Schweizer HP.
TITLE Direct Submission
JOURNAL Submitted (06-APR-1999) Microbiology, Colorado State University,
Fort Collins, CO 80523, USA
REFERENCE 3 (bases 1 to 6370)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6370)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(06-APR-1999) Microbiology, Colorado State University, Fort Collins,
CO 80523, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6370
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 827..845
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind 882..898
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(911..1624)
/codon_start=1
/label=GFP
/note="Aequorea victoria green fluorescent protein"
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
primer_bind complement(1692..1708)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(1734..1752)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind complement(1926..1973)
/label=FRT
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
oriT complement(2180..2288)
/direction=LEFT
/label=oriT
/note="incP origin of transfer"
rep_origin complement(3713..4301)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5126..6313)
/codon_start=1
/label=TcR
/note="tetracycline efflux protein"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
promoter complement(join(6361..6370,1..19))
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
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