Basic Vector Information
- Vector Name:
- LR_UAS-EYFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7209 bp
- Type:
- Mammalian expression vector
- Replication origin:
- ori
- Source/Author:
- Siciliano V.
LR_UAS-EYFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
LR_UAS-EYFP vector Sequence
LOCUS 40924_1849 7209 bp DNA circular SYN 17-DEC-2018
DEFINITION Mammalian expression vector LR_UAS-EYFP, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7209)
AUTHORS Siciliano V.
TITLE Engineering modular intracellular protein sensor-actuator devices
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7209)
AUTHORS Siciliano V.
TITLE Direct Submission
JOURNAL Submitted (07-APR-2018) Biological Engineering, Massachusetts
Institute of Technology, 500 Tech Square, Cambridge, MA 02139, USA
REFERENCE 3 (bases 1 to 7209)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7209)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(07-APR-2018) Biological Engineering, Massachusetts Institute of
Technology, 500 Tech Square, Cambridge, MA 02139, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7209
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(160..1017)
/label=AmpR
/note="beta-lactamase"
promoter complement(1018..1122)
/label=AmpR promoter
rep_origin complement(1148..1603)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 1745..1761
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 1771..1789
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 1804..2607
/label=HA-L
/note="left homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
misc_feature 2686..2711
/label=SA
/note="splice acceptor site"
protein_bind 3383..3403
/label=attB4
/note="core recombination site for the Gateway(R) BP
reaction"
regulatory 3448..3600
/regulatory_class="promoter"
protein_bind 3623..3647
/label=attB1
/note="recombination site for the Gateway(R) BP reaction"
CDS 3674..4390
/label=EYFP
/note="enhanced YFP"
protein_bind complement(4418..4442)
/label=attB2
/note="recombination site for the Gateway(R) BP reaction"
polyA_signal 4597..4652
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(5013..5029)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 5037..5053
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5061..5091)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5106..5127
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 5300..6136
/label=HA-R
/note="right homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
promoter complement(6166..6184)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(6205..6221)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(6229..6245)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6253..6283)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(6298..6319)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6607..7195)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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