LMP-1 vector (V009861)

Basic Vector Information

      • Vector Name:
      • LMP-1
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 5527 bp
      • Type:
      • Expression vector
      • Source/Author:
      • Lee J, Sugden B.

LMP-1 vector Vector Map

LMP-15527 bp60012001800240030003600420048005400SV40 promoter-beta-globin intronT7LMP-1SV40 poly(A) signaloriblaAmpR promoterf1 oriM13 fwd

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

LMP-1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                5527 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector LMP-1, complete sequence.
ACCESSION   HQ706129
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Lee J, Sugden B.
  TITLE     A membrane leucine heptad contributes to trafficking, signaling, and
            transformation by latent membrane protein 1
  JOURNAL   J. Virol. 81 (17), 9121-9130 (2007)
  PUBMED    17581993
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Lee J, Sugden B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2010) Department of Oncology, McArdle Laboratory 
            for Cancer Research, University of Wisconsin-Madison, 1400 
            University Ave, Madison, WI 53705, USA
REFERENCE   3  (bases 1 to 5527)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Expression vector LMP-1"
                     /mol_type="other DNA"
                     /db_xref="taxon:984255"
     promoter        9..366
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      217..352
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          395..967
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1022..1040
                     /regulatory_class="promoter"
                     /note="T7"
     promoter        1022..1040
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1066..2226
                     /codon_start=1
                     /product="LMP-1"
                     /label=LMP-1
                     /protein_id="ADX86837.1"
                     /translation="MEHDLERGPPGPRRPPRGPPLSSSLGLALLLLLLALLFWLYIVMS
                     DWTGGALLVLYSFALMLIIIILIIFIFRRDLLCPLGALCILLLMITLLLIALWNLHGQA
                     LYLGIVLFIFGCLLVLGLWIYLLEILWRLGATIWQLLAFFLAFFLDLILLIIALYLQQN
                     WWTLLVDLLWLLLFLAILIWMYYHGQRHSDEHHHDDSLPHPQQATDDSGHESDSNSNEG
                     RHHLLVSGAGDGPPLCSQNLGAPGGGPDNGPQDPDNTDDNGPQDPDNTDDNGPHDPLPQ
                     DPDNTDDNGPQDPDNTDDNGPHDPLPHSPSDSAGNDGGPPQLTEEVENKGGDQGPPLMT
                     DGGGGHSHDSGHGGGDPHLPTLLLGSSGSGGDDDDPHGPVQLSYYD"
     regulatory      2118..2127
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     polyA_signal    2523..2644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2849..3437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3608..4468)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance"
                     /protein_id="ADX86836.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3608..4468)
                     /gene="bla"
                     /label=bla
     promoter        complement(4469..4573)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4906..5361)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5506..5522
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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