LMP-1 vector (V009861)

MSDS

Basic Vector Information

Vector Name:: LMP-1

Antibiotic Resistance:: Ampicillin

Length:: 5527 bp

Type:: Expression vector

Replication origin:: ori

Source/Author:: Lee J, Sugden B.

LMP-1 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

LMP-1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_1829        5527 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector LMP-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Lee J, Sugden B.
  TITLE     A membrane leucine heptad contributes to trafficking, signaling, and
            transformation by latent membrane protein 1
  JOURNAL   J. Virol. 81 (17), 9121-9130 (2007)
  PUBMED    17581993
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Lee J, Sugden B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2010) Department of Oncology, McArdle Laboratory 
            for Cancer Research, University of Wisconsin-Madison, 1400 
            University Ave, Madison, WI 53705, USA
REFERENCE   3  (bases 1 to 5527)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5527)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.";
            date: "2007"; volume: "81"; issue: "17"; pages: "9121-9130"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-DEC-2010) Department of Oncology, McArdle Laboratory for Cancer 
            Research, University of Wisconsin-Madison, 1400 University Ave, 
            Madison, WI 53705, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..366
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          395..967
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        1022..1040
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1066..2223
                     /gene="LMP1"
                     /label=LMP1
                     /note="Latent membrane protein 1 from Epstein-Barr virus
                     (strain B95-8). Accession#: P03230"
     polyA_signal    2523..2644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2849..3437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3611..4468)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4469..4573)
                     /label=AmpR promoter
     rep_origin      complement(4906..5361)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5506..5522
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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