L1R1_neo vector (V009882)

Basic Vector Information

      • Vector Name:
      • L1R1_neo
      • Antibiotic Resistance:
      • Kanamycin
      • Length:
      • 10540 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Drozd S.

L1R1_neo vector Vector Map

L1R1_neo10540 bp5001000150020002500300035004000450050005500600065007000750080008500900095001000010500T3 promoterSV40 poly(A) signalf1 oriSV40 promoterNeoR/KanRsynthetic polyadenylation signalAmpR promoterblaoriCMV enhancerCMV promoterchimeric intronT7 promoterSV40 poly(A) signallacZ

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

L1R1_neo vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported               10540 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector L1R1_neo, complete sequence.
ACCESSION   KT600161
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10540)
  AUTHORS   Drozd S.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2015) Koltzov Institute of Developmental Biology, 
            Russian Academy of Sciences, ul. Vavilova 26, Moscow 119334, Russia
REFERENCE   2  (bases 1 to 10540)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10540
                     /organism="Cloning vector L1R1_neo"
                     /mol_type="other DNA"
                     /label=based on the Promega vector pCI-neo
                     /note="based on the Promega vector pCI-neo"
                     /db_xref="taxon:1708077"
     promoter        complement(10..27)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    45..166
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      352..807
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        924..1281
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1132..1267
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1332..2126
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    2190..2238
                     /note="synthetic polyadenylation signal"
     promoter        2544..2648
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2649..3509
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ALC74888.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            2649..3509
                     /gene="bla"
                     /label=bla
     rep_origin      3680..4268
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        4479..4858
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4859..5070
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          5231..5363
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        5408..5426
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5856..5990
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6255..9326)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="TMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTD
                     RPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVT
                     YPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQ
                     DSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQI
                     SDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDE
                     RGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENG
                     LLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWY
                     TLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSL
                     GNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPK
                     WSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLI
                     KYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQT
                     IEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQL
                     WLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGN
                     KRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKA
                     AGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVE
                     VASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYV
                     FPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGF
                     HMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     9293..9309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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