Basic Vector Information
ipSilent vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
ipSilent vector Sequence
LOCUS 40924_1414 6086 bp DNA circular SYN 17-DEC-2018 DEFINITION Gene silencing vector ipSilent DNA, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6086) AUTHORS Vu BV, Pham KT, Nakayashiki H. TITLE Substrate-Induced Transcriptional Activation of the MoCel7C Cellulase Gene Is Associated with Methylation of Histone H3 at Lysine 4 in the Rice Blast Fungus Magnaporthe oryzae JOURNAL Appl. Environ. Microbiol. 79 (21), 6823-6832 (2013) PUBMED 23995923 REFERENCE 2 (bases 1 to 6086) AUTHORS Nakayashiki H, Vu BV. TITLE Direct Submission JOURNAL Submitted (22-SEP-2012) Contact:Hitoshi Nakayashiki Kobe University, Faculty of Agriculture; 1-1 Rokkodai, Nada, Kobe, Hyogo 657-8501, Japan REFERENCE 3 (bases 1 to 6086) TITLE Direct Submission REFERENCE 4 (bases 1 to 6086) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Appl. Environ. Microbiol."; date: "2013"; volume: "79"; issue: "21"; pages: "6823-6832" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (22-SEP-2012) Contact:Hitoshi Nakayashiki Kobe University, Faculty of Agriculture; 1-1 Rokkodai, Nada, Kobe, Hyogo 657-8501, Japan" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..6086 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 24..128 /label=AmpR promoter CDS 129..986 /label=AmpR /note="beta-lactamase" rep_origin 1160..1748 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2036..2057 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 2072..2102 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2110..2126 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2134..2150 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 2171..2189 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 2234..2588 /label=trpC promoter /note="promoter for Aspergillus nidulans trpC" CDS 2593..3615 /label=HygR /note="aminoglycoside phosphotransferase from E. coli" regulatory 3619..3864 /label=Aspergillus nidulans TrpC terminator /note="Aspergillus nidulans TrpC terminator" /regulatory_class="terminator" primer_bind 3873..3889 /label=SK primer /note="common sequencing primer, one of multiple similar variants" regulatory 3892..4990 /label=Magnaporthe oryzae MoCel7C promoter /note="Magnaporthe oryzae MoCel7C promoter" /regulatory_class="promoter" misc_feature 4991..5028 /label=multi-cloning sites /note="multi-cloning sites" intron 5029..5162 /note="Magnaporthe oryzae cutinase intron2" misc_feature 5163..5228 /label=multi-cloning sites /note="multi-cloning sites" regulatory 5229..5432 /label=Magnaporthe oryzae MoCel7C terminator /note="Magnaporthe oryzae MoCel7C terminator" /regulatory_class="terminator" promoter complement(5443..5461) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(5471..5487) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 5629..6084 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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