ipSilent vector (V009905)

Basic Vector Information

      • Vector Name:
      • ipSilent
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 6086 bp
      • Type:
      • Gene silencing vector
      • Source/Author:
      • Vu BV, Pham KT, Nakayashiki H.

ipSilent vector Vector Map

ipSilent6086 bp30060090012001500180021002400270030003300360039004200450048005100540057006000AmpR promoterAmpRoriCAP binding sitelac promoterlac operatorM13 revT3 promoterAspergillus nidulans TrpC promoterHygrAspergillus nidulans TrpC terminatorSK primerMagnaporthe oryzae MoCel7C promotermulti-cloning sitesMagnaporthe oryzae cutinase intron2multi-cloning sitesMagnaporthe oryzae MoCel7C terminatorT7 promoterM13 fwdf1 ori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

ipSilent vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                6086 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Gene silencing vector ipSilent DNA, complete sequence.
ACCESSION   AB750393
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Vu BV, Pham KT, Nakayashiki H.
  TITLE     Substrate-Induced Transcriptional Activation of the MoCel7C 
            Cellulase Gene Is Associated with Methylation of Histone H3 at 
            Lysine 4 in the Rice Blast Fungus Magnaporthe oryzae
  JOURNAL   Appl. Environ. Microbiol. 79 (21), 6823-6832 (2013)
  PUBMED    23995923
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Nakayashiki H, Vu BV.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2012) Contact:Hitoshi Nakayashiki Kobe University,
            Faculty of Agriculture; 1-1 Rokkodai, Nada, Kobe, Hyogo 657-8501, 
            Japan
REFERENCE   3  (bases 1 to 6086)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Gene silencing vector ipSilent"
                     /mol_type="other DNA"
                     /note="ipSilent, inducible gene silencing vector for 
                     ascomycetous fungi"
                     /db_xref="taxon:1234704"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             129..989
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      2226..2592
                     /regulatory_class="promoter"
                     /note="Aspergillus nidulans TrpC promoter"
     CDS             2593..3618
                     /codon_start=1
                     /gene="Hygr"
                     /product="hygromycin resistance gene"
                     /label=Hygr
                     /protein_id="BAM45085.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     gene            2593..3618
                     /gene="Hygr"
                     /label=Hygr
     regulatory      3619..3864
                     /regulatory_class="terminator"
                     /note="Aspergillus nidulans TrpC terminator"
     primer_bind     3873..3889
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      3892..4990
                     /regulatory_class="promoter"
                     /note="Magnaporthe oryzae MoCel7C promoter"
     misc_feature    4991..5028
                     /label=multi-cloning sites
                     /note="multi-cloning sites"
     intron          5029..5162
                     /note="Magnaporthe oryzae cutinase intron2"
     misc_feature    5163..5228
                     /label=multi-cloning sites
                     /note="multi-cloning sites"
     regulatory      5229..5432
                     /regulatory_class="terminator"
                     /note="Magnaporthe oryzae MoCel7C terminator"
     promoter        complement(5443..5461)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5471..5487)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5629..6084
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"

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