Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V009906 | I-SceI-pBSII-SK+ | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
- Vector Name:
- I-SceI-pBSII-SK+
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3007 bp
- Type:
- Transgenesis vector
- Replication origin:
- ori
- Source/Author:
- Thermes V, Grabher C, Ristoratore F, Bourrat F, Choulika A, Wittbrodt J, Joly JS.
- Growth Strain(s):
- TOP10
- Growth Temperature:
- 37℃
I-SceI-pBSII-SK+ vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
I-SceI-pBSII-SK+ vector Sequence
LOCUS 40924_1394 3007 bp DNA circular SYN 17-DEC-2018
DEFINITION Transgenesis vector I-SceI-pBSII-SK+, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3007)
AUTHORS Thermes V, Grabher C, Ristoratore F, Bourrat F, Choulika A,
Wittbrodt J, Joly JS.
TITLE I-SceI meganuclease mediates highly efficient transgenesis in fish
JOURNAL Mech. Dev. 118 (1-2), 91-98 (2002)
PUBMED 12351173
REFERENCE 2 (bases 1 to 3007)
AUTHORS Wittbrodt J, Rembold M.
TITLE Direct Submission
JOURNAL Submitted (04-JUL-2006) Developmental Biology Unit, EMBL Heidelberg,
Meyerhofstrasse 1, Heidelberg 69117, Germany
REFERENCE 3 (bases 1 to 3007)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3007)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mech. Dev.
118 (1-2), 91-98 (2002)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(04-JUL-2006) Developmental Biology Unit, EMBL Heidelberg,
Meyerhofstrasse 1, Heidelberg 69117, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3007
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(3..458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 600..616
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 624..641
/label=I-SceI site
/note="I-SceI site"
promoter 649..667
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature complement(676..783)
/label=MCS
/note="pBluescript multiple cloning site"
promoter complement(796..814)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
misc_feature 821..838
/label=I-SceI site
/note="I-SceI site"
primer_bind complement(858..874)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(882..898)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(906..936)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(951..972)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(1260..1848)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2022..2879)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2880..2984)
/label=AmpR promoter