I-SceI-pBSII-SK+ vector (V009906)

Price Information

Cat No. Plasmid Name Availability Add to cart
V009906 I-SceI-pBSII-SK+ In stock (lyophilized plasmid)

Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

      • Vector Name:
      • I-SceI-pBSII-SK+
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 3007 bp
      • Type:
      • Transgenesis vector
      • Source/Author:
      • Thermes V, Grabher C, Ristoratore F, Bourrat F, Choulika A, Wittbrodt J, Joly JS.
      • Growth Strain(s):
      • TOP10
      • Growth Temperature:
      • 37℃

I-SceI-pBSII-SK+ vector Vector Map

I-SceI-pBSII-SK+3007 bp6001200180024003000f1 oriM13 fwdI-SceI siteT7 promoterMCST3 promoterI-SceI siteM13 revlac operatorlac promoterCAP binding siteoribeta-lactamaseAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

I-SceI-pBSII-SK+ vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                3007 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Transgenesis vector I-SceI-pBSII-SK+, complete sequence.
ACCESSION   DQ836146
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Thermes V, Grabher C, Ristoratore F, Bourrat F, Choulika A, 
            Wittbrodt J, Joly JS.
  TITLE     I-SceI meganuclease mediates highly efficient transgenesis in fish
  JOURNAL   Mech. Dev. 118 (1-2), 91-98 (2002)
  PUBMED    12351173
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Wittbrodt J, Rembold M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUL-2006) Developmental Biology Unit, EMBL Heidelberg,
            Meyerhofstrasse 1, Heidelberg 69117, Germany
REFERENCE   3  (bases 1 to 3007)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Transgenesis vector I-SceI-pBSII-SK+"
                     /mol_type="other DNA"
                     /db_xref="taxon:396799"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    624..641
                     /label=I-SceI site
                     /note="I-SceI site"
     promoter        649..667
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    676..783
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     misc_feature    677..783
                     /note="MCS; multiple cloning site"
     primer_bind     693..709
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(743..759)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(796..814)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    821..838
                     /label=I-SceI site
                     /note="I-SceI site"
     primer_bind     complement(858..874)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    882..898
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(906..936)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..972
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1260..1848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2019..2879)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="ABH04321.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2880..2984)
                     /gene="bla"
                     /label=AmpR promoter