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Basic Vector Information
Basic Vector Information | |||
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Vector Name | HO-hisG-URA3-hisG-poly-HO | Antibiotic Resistance | Ampicillin |
Length | 8487 bp | Type | Cloning vector |
Source | Voth WP, Richards JD, Shaw JM, Stillman DJ. |
HO-hisG-URA3-hisG-poly-HO vector Vector Map
HO-hisG-URA3-hisG-poly-HO vector Sequence
LOCUS Exported 8487 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector HO-hisG-URA3-hisG-poly-HO, complete sequence. ACCESSION AF324729 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8487) AUTHORS Voth WP, Richards JD, Shaw JM, Stillman DJ. TITLE Yeast vectors for integration at the HO locus JOURNAL Nucleic Acids Res. 29 (12), E59 (2001) PUBMED 11410682 REFERENCE 2 (bases 1 to 8487) AUTHORS Voth WP, Richards J, Shaw J, Stillman DJ. TITLE Direct Submission JOURNAL Submitted (29-NOV-2000) Pathology, University of Utah, 50 N. Medical Drive, 5C124 SOM, Salt Lake City, UT 84132, USA REFERENCE 3 (bases 1 to 8487) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 18, 2018 from SnapGene 4.1.8 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8487 /organism="Cloning vector HO-hisG-URA3-hisG-poly-HO" /mol_type="genomic DNA" /db_xref="taxon:153591" misc_feature 1..8487 /note="vector designed for targeted integration to the HO locus in yeast; plasmid contains two fragments from the HO promoter inserted in the pUC21-NotI polylinker, along with the hisG-URA3-hisG selectable marker; HO region is bracketed by two NotI sites, with polylinker sites between the HO and hisG-URA3-hisG regions" promoter 2199..2414 /gene="S. cerevisiae URA3" /label=URA3 promoter CDS 2415..3218 /codon_start=1 /gene="S. cerevisiae URA3" /product="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" /label=URA3 /note="yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" primer_bind 5420..5436 /label=SK primer /note="common sequencing primer, one of multiple similar variants" promoter complement(5451..5469) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(5476..5492) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 6364..6468 /gene="bla" /label=AmpR promoter CDS 6469..7329 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 7500..8088 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 8376..8397 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." protein_bind 8452..8468 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8476..5 /label=M13 rev /note="common sequencing primer, one of multiple similar variants"