Basic Vector Information
- Vector Name:
- HO-hisG-URA3-hisG-poly-HO
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8487 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Voth WP, Richards JD, Shaw JM, Stillman DJ.
- Promoter:
- URA3
HO-hisG-URA3-hisG-poly-HO vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
HO-hisG-URA3-hisG-poly-HO vector Sequence
LOCUS 40924_1319 8487 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector HO-hisG-URA3-hisG-poly-HO, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8487)
AUTHORS Voth WP, Richards JD, Shaw JM, Stillman DJ.
TITLE Yeast vectors for integration at the HO locus
JOURNAL Nucleic Acids Res. 29 (12), E59 (2001)
PUBMED 11410682
REFERENCE 2 (bases 1 to 8487)
AUTHORS Voth WP, Richards J, Shaw J, Stillman DJ.
TITLE Direct Submission
JOURNAL Submitted (29-NOV-2000) Pathology, University of Utah, 50 N. Medical
Drive, 5C124 SOM, Salt Lake City, UT 84132, USA
REFERENCE 3 (bases 1 to 8487)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8487)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
Acids Res."; date: "2001"; volume: "29"; issue: "12"; pages: "E59"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(29-NOV-2000) Pathology, University of Utah, 50 N. Medical Drive,
5C124 SOM, Salt Lake City, UT 84132, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8487
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 1112..2008
/gene="hisG"
/label=hisG
/note="ATP phosphoribosyltransferase from Salmonella
heidelberg (strain SL476). Accession#: B4T9N3"
promoter 2194..2414
/label=URA3 promoter
CDS 2415..3215
/label=URA3
/note="orotidine-5'-phosphate decarboxylase, required for
uracil biosynthesis"
CDS 3815..4711
/gene="hisG"
/label=hisG
/note="ATP phosphoribosyltransferase from Salmonella
heidelberg (strain SL476). Accession#: B4T9N3"
primer_bind 5420..5436
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(5451..5469)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5476..5492)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 6364..6468
/label=AmpR promoter
CDS 6469..7326
/label=AmpR
/note="beta-lactamase"
rep_origin 7500..8088
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 8376..8397
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
protein_bind 8452..8468
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
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