HO-hisG-URA3-hisG-poly-HO vector (V009920)

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LOCUS       V009920                 8487 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009920
VERSION     V009920
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8487)
  AUTHORS   Voth WP, Richards JD, Shaw JM, Stillman DJ.
  TITLE     Yeast vectors for integration at the HO locus
  JOURNAL   Nucleic Acids Res. 29 (12), E59 (2001)
   PUBMED   11410682
REFERENCE   2  (bases 1 to 8487)
  AUTHORS   Voth WP, Richards J, Shaw J, Stillman DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2000) Pathology, University of Utah, 50 N. Medical
            Drive, 5C124 SOM, Salt Lake City, UT 84132, USA
REFERENCE   3  (bases 1 to 8487)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8487)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "2001"; volume: "29"; issue: "12"; pages: "E59"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-NOV-2000) Pathology, University of Utah, 50 N. Medical Drive,
            5C124 SOM, Salt Lake City, UT 84132, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1112..2008
                     /gene="hisG"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Salmonella
                     heidelberg (strain SL476). Accession#: B4T9N3"
     promoter        2194..2414
                     /label="URA3 promoter"
     CDS             2415..3215
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     CDS             3815..4711
                     /gene="hisG"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Salmonella
                     heidelberg (strain SL476). Accession#: B4T9N3"
     primer_bind     5420..5436
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(5451..5469)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5476..5492)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6364..6468
                     /label="AmpR promoter"
     CDS             6469..7326
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7500..8088
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8376..8397
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     protein_bind    8452..8468
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."