HBT-sGFP(S65T)-NOS vector (V009925)

Price Information

Cat No. Plasmid Name Availability Add to cart
V009925 HBT-sGFP(S65T)-NOS In stock (lyophilized plasmid)

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Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

The HBT-sGFP(S65T)-NOS is a transient expression vector. FPs maturation times and fluorescent intensity can be affected by the temperature. For instance, enhanced GFP (EGFP) was optimized for 37°C, and is therefore most suited for mammalian or bacteria studies, whereas GFPS65T is better suited for yeast studies (24-30°C).

      • Vector Name:
      • HBT-sGFP(S65T)-NOS
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 4230 bp
      • Type:
      • Control vector
      • Source/Author:
      • Sheen J.
      • Growth Strain(s):
      • Stbl3
      • Growth Temperature:
      • 37℃

HBT-sGFP(S65T)-NOS vector Vector Map

HBT-sGFP(S65T)-NOS4230 bp600120018002400300036004200pUC18CMV35s promotersGFPNOS terminator

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

  • Zhao W, Geng H, Dan Z, Zeng Y, Wang M, Xu W, Hu Z, Huang W. The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS Rice. Int J Mol Sci. 2023 Mar 13;24(6):5442.

HBT-sGFP(S65T)-NOS vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                4230 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Control vector HBT-sGFP(S65T)-NOS, complete sequence.
ACCESSION   EF090408
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Sheen J.
  TITLE     Protein phosphatase activity is required for light-inducible gene 
            expression in maize
  JOURNAL   EMBO J. 12 (9), 3497-3505 (1993)
  PUBMED    8253076
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Chiu W, Niwa Y, Zeng W, Hirano T, Kobayashi H, Sheen J.
  TITLE     Engineered GFP as a vital reporter in plants
  JOURNAL   Curr. Biol. 6 (3), 325-330 (1996)
  PUBMED    8805250
REFERENCE   3  (bases 1 to 4230)
  AUTHORS   Sheen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) Molecular Biology, Massachusetts General 
            Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, 
            Boston, MA 02114, USA
REFERENCE   4  (bases 1 to 4230)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Control vector HBT-sGFP(S65T)-NOS"
                     /mol_type="other DNA"
                     /label=derived from Arabidopsis thaliana
                     /note="derived from Arabidopsis thaliana"
                     /db_xref="taxon:412978"
     misc_feature    25..2663
                     /label=pUC18
                     /note="pUC18"
     primer_bind     complement(39..55)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    63..79
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(87..117)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    132..153
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(441..1029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1200..2060)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2061..2165)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2639..2655
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2675..3019
                     /regulatory_class="promoter"
                     /note="CMV35s promoter"
     promoter        2722..3231
                     /label=35SPPDK promoter
                     /note="hybrid promoter consisting of the cauliflower mosaic
                     virus 35S enhancer fused to the maize C4PPDK basal promoter
                     (Yoo et al., 2007)"
     regulatory      3044..3231
                     /regulatory_class="promoter"
                     /note="C4ppdk1Zm"
     CDS             3240..3959
                     /codon_start=1
                     /product="sGFP"
                     /label=sGFP
                     /note="S65T"
                     /protein_id="ABL09837.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     terminator      3978..4230
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      3981..4230
                     /regulatory_class="terminator"
                     /note="NOS terminator"