Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V009925 | HBT-sGFP(S65T)-NOS | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
The HBT-sGFP(S65T)-NOS is a transient expression vector. FPs maturation times and fluorescent intensity can be affected by the temperature. For instance, enhanced GFP (EGFP) was optimized for 37°C, and is therefore most suited for mammalian or bacteria studies, whereas GFPS65T is better suited for yeast studies (24-30°C).
HBT-sGFP(S65T)-NOS vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Zhao W, Geng H, Dan Z, Zeng Y, Wang M, Xu W, Hu Z, Huang W. The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS Rice. Int J Mol Sci. 2023 Mar 13;24(6):5442.
HBT-sGFP(S65T)-NOS vector Sequence
LOCUS 40924_1279 4230 bp DNA circular SYN 17-DEC-2018
DEFINITION Control vector HBT-sGFP(S65T)-NOS, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4230)
AUTHORS Sheen J.
TITLE Protein phosphatase activity is required for light-inducible gene
expression in maize
JOURNAL EMBO J. 12 (9), 3497-3505 (1993)
PUBMED 8253076
REFERENCE 2 (bases 1 to 4230)
AUTHORS Chiu W, Niwa Y, Zeng W, Hirano T, Kobayashi H, Sheen J.
TITLE Engineered GFP as a vital reporter in plants
JOURNAL Curr. Biol. 6 (3), 325-330 (1996)
PUBMED 8805250
REFERENCE 3 (bases 1 to 4230)
AUTHORS Sheen J.
TITLE Direct Submission
JOURNAL Submitted (27-OCT-2006) Molecular Biology, Massachusetts General
Hospital, 185 Cambridge St. Simches Research Building-CPZN7600,
Boston, MA 02114, USA
REFERENCE 4 (bases 1 to 4230)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 4230)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J.";
date: "1993"; volume: "12"; issue: "9"; pages: "3497-3505"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Curr.
Biol."; date: "1996"; volume: "6"; issue: "3"; pages: "325-330"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185
Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114,
USA"
COMMENT SGRef: number: 4; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4230
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(39..55)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(63..79)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(87..117)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(132..153)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(441..1029)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1203..2060)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2061..2165)
/label=AmpR promoter
primer_bind 2639..2655
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 2722..3231
/label=35SPPDK promoter
/note="hybrid promoter consisting of the cauliflower mosaic
virus 35S enhancer fused to the maize C4PPDK basal promoter
(Yoo et al., 2007)"
CDS 3240..3956
/codon_start=1
/label=EGFP
/note="enhanced GFP"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITHGMDELYK"
terminator 3978..4230
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"