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GmubiSINCTIF1-pFLEV vector

Basic Vector Information

Basic Vector Information
Vector Name GmubiSINCTIF1-pFLEV Antibiotic Resistance Ampicillin
Length 4405 bp Type Cloning vector
Source Grant TN, De La Torre CM, Zhang N, Finer JJ.

GmubiSINCTIF1-pFLEV vector Vector Map

GmubiSINCTIF1-pFLEV vector Sequence

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LOCUS       Exported                4405 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmubiSINCTIF1-pFLEV, complete sequence.
ACCESSION   KX814446
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Grant TN, De La Torre CM, Zhang N, Finer JJ.
  TITLE     Synthetic introns help identify sequences in the 5' UTR intron of 
            the Glycine max polyubiquitin (Gmubi) promoter that give increased 
            promoter activity
  JOURNAL   Planta (2017) In press
  PUBMED    28070655
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Finer J, Grant T, De La Torre C, Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 4405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 18, 2018 from SnapGene 4.1.8
            http://www.snapgene.com
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Cloning vector GmubiSINCTIF1-pFLEV"
                     /mol_type="other DNA"
                     /db_xref="taxon:1955485"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      412..1151
                     /regulatory_class="promoter"
                     /note="GmubiSINCTIF1 synthetic promoter"
     CDS             1152..1871
                     /codon_start=1
                     /product="sGFP65T"
                     /label=sGFP65T
                     /protein_id="AQM73600.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     regulatory      1890..2165
                     /regulatory_class="terminator"
                     /note="NOS terminator"
     terminator      1890..2142
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2184..2200)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2208..2224
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2232..2262)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2277..2298
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2586..3174)
                     /direction=LEFT
     CDS             complement(3345..4205)
                     /codon_start=1
                     /gene="ampR"
                     /product="ampicillin resistance protein"
                     /label=ampR
                     /protein_id="AQM73599.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3345..4205)
                     /gene="ampR"
                     /label=ampR
     promoter        complement(4206..4310)
                     /gene="bla"
                     /label=AmpR promoter

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