GCC1-LUC-NOS vector (V009958)

LOCUS       40924_1049        4854 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Control vector GCC1-LUC-NOS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Yanagisawa S, Yoo SD, Sheen J.
  TITLE     Differential regulation of EIN3 stability by glucose and ethylene 
            signalling in plants
  JOURNAL   Nature 425 (6957), 521-525 (2003)
  PUBMED    14523448
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Sheen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) Molecular Biology, Massachusetts General 
            Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, 
            Boston, MA 02114, USA
REFERENCE   3  (bases 1 to 4854)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4854)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2003"; volume: "425"; issue: "6957"; pages: "521-525"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185
            Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(15..31)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(39..55)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(63..93)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(108..129)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(417..1005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1179..2036)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2037..2142)
                     /label=AmpR promoter
     primer_bind     2616..2632
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2642..2690
                     /label=8*GCC enhancer
                     /note="8*GCC enhancer"
                     /regulatory_class="enhancer"
     promoter        2722..2768
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             2791..4440
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      4598..4849
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"