Basic Vector Information
- Vector Name:
- GCC1-LUC-NOS
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4854 bp
- Type:
- Control vector
- Replication origin:
- ori
- Source/Author:
- Yanagisawa S, Yoo SD, Sheen J.
GCC1-LUC-NOS vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
GCC1-LUC-NOS vector Sequence
LOCUS 40924_1049 4854 bp DNA circular SYN 17-DEC-2018
DEFINITION Control vector GCC1-LUC-NOS, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4854)
AUTHORS Yanagisawa S, Yoo SD, Sheen J.
TITLE Differential regulation of EIN3 stability by glucose and ethylene
signalling in plants
JOURNAL Nature 425 (6957), 521-525 (2003)
PUBMED 14523448
REFERENCE 2 (bases 1 to 4854)
AUTHORS Sheen J.
TITLE Direct Submission
JOURNAL Submitted (27-OCT-2006) Molecular Biology, Massachusetts General
Hospital, 185 Cambridge St. Simches Research Building-CPZN7600,
Boston, MA 02114, USA
REFERENCE 3 (bases 1 to 4854)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4854)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nature";
date: "2003"; volume: "425"; issue: "6957"; pages: "521-525"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185
Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114,
USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4854
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(15..31)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(39..55)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(63..93)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(108..129)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(417..1005)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1179..2036)
/label=AmpR
/note="beta-lactamase"
promoter complement(2037..2142)
/label=AmpR promoter
primer_bind 2616..2632
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
regulatory 2642..2690
/label=8*GCC enhancer
/note="8*GCC enhancer"
/regulatory_class="enhancer"
promoter 2722..2768
/label=minimal CaMV 35S promoter
/note="minimal 35S promoter from cauliflower mosaic virus"
CDS 2791..4440
/label=luciferase
/note="firefly luciferase"
terminator 4598..4849
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
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