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GCC1-LUC-NOS vector (V009958#)

Basic Vector Information

Basic Vector Information
Vector Name GCC1-LUC-NOS Antibiotic Resistance Ampicillin
Length 4854 bp Type Control vector
Source Yanagisawa S, Yoo SD, Sheen J.

GCC1-LUC-NOS vector Vector Map

cloning vector 8*GCC enhancer 35 minimum promoter luciferase NOS terminator

GCC1-LUC-NOS vector Sequence

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Download GeneBank File(.gb)

LOCUS       Exported                4854 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Control vector GCC1-LUC-NOS, complete sequence.
ACCESSION   EF090415
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Yanagisawa S, Yoo SD, Sheen J.
  TITLE     Differential regulation of EIN3 stability by glucose and ethylene 
            signalling in plants
  JOURNAL   Nature 425 (6957), 521-525 (2003)
  PUBMED    14523448
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Sheen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) Molecular Biology, Massachusetts General 
            Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, 
            Boston, MA 02114, USA
REFERENCE   3  (bases 1 to 4854)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Control vector GCC1-LUC-NOS"
                     /mol_type="other DNA"
                     /label=derived from Arabidopsis thaliana
                     /note="derived from Arabidopsis thaliana"
                     /db_xref="taxon:412985"
     misc_feature    1..2641
                     /label=cloning vector
                     /note="cloning vector"
     primer_bind     complement(15..31)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(63..93)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    108..129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(417..1005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1176..2036)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2037..2142)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2616..2632
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2642..2690
                     /regulatory_class="enhancer"
                     /note="8*GCC enhancer"
     regulatory      2691..2790
                     /regulatory_class="promoter"
                     /note="35 minimum promoter"
     promoter        2722..2768
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             2791..4443
                     /codon_start=1
                     /product="luciferase"
                     /label=luciferase
                     /note="LUC"
                     /protein_id="ABL09844.1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     terminator      4598..4849
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      4601..4849
                     /regulatory_class="terminator"
                     /note="NOS terminator"

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