GAPTrapT2AIresMuro vector (V009962)

Basic Vector Information

      • Vector Name:
      • GAPTrapT2AIresMuro
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 11810 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E, Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Peningt

GAPTrapT2AIresMuro vector Vector Map

GAPTrapT2AIresMuro11810 bp50010001500200025003000350040004500500055006000650070007500800085009000950010000105001100011500ampR

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

GAPTrapT2AIresMuro vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported               11810 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GAPTrapT2AIresMuro, complete sequence.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11810)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG.
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives
  JOURNAL   Stem Cell Reports (2016) In press
  PUBMED    27594589
REFERENCE   2  (bases 1 to 11810)
  AUTHORS   Stanley E.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2016) Cell Biology, Murdoch Childrens Research 
            Institute, Royal Children's Hospital, Flemington Road, Melbourne, 
            Victoria 3052, Australia
REFERENCE   3  (bases 1 to 11810)
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
FEATURES             Location/Qualifiers
     source          1..11810
                     /organism="Cloning vector GAPTrapT2AIresMuro"
                     /mol_type="other DNA"
     gene            24..989
     regulatory      24..128
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..989
     rep_origin      1160..11748
     rep_origin      1160..1748
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
     protein_bind    2036..2057
                     /label=CAP binding site
     regulatory      2072..2102
                     /note="lac promoter"
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     misc_feature    2110..2126
                     /label=lac operator
                     /note="lac operator"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="M13 rev"
     misc_feature    2146..2200
     regulatory      2171..2189
                     /note="T3 promoter"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     gene            2216..5559
                     /note="glyceraldehyde-3-phosphate dehydrogenase"
     misc_feature    2219..5556
                     /label=left arm
                     /note="left arm"
     misc_feature    5560..5658
     CDS             5581..5634
                     /product="2A peptide from Thosea asigna virus capsid 
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
     misc_feature    5657..6243
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     regulatory      5684..6246
     CDS             6253..6861
                     /note="codes for puromycin resistance"
     misc_feature    6897..11132
                     /label=right arm
                     /note="right arm"
     misc_feature    11152..11810
                     /label=pBluescript II KS(-)
                     /note="pBluescript II KS(-)"
     promoter        complement(11167..11185)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11195..11211)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
     rep_origin      complement(11352..11807)
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"

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