Basic Vector Information
- Vector Name:
- Ganesh-Z1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 14142 bp
- Type:
- Reporter vector
- Replication origin:
- ori
- Source/Author:
- Swanson CI, Hinrichs T, Johnson LA, Zhao Y, Barolo S.
Ganesh-Z1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Ganesh-Z1 vector Sequence
LOCUS 40924_1019 14142 bp DNA circular SYN 17-DEC-2018
DEFINITION Reporter vector Ganesh-Z1, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14142)
AUTHORS Swanson CI, Hinrichs T, Johnson LA, Zhao Y, Barolo S.
TITLE A directional recombination cloning system for restriction- and
ligation-free construction of GFP, DsRed, and lacZ transgenic
Drosophila reporters
JOURNAL Gene 408 (1-2), 180-186 (2008)
PUBMED 18077106
REFERENCE 2 (bases 1 to 14142)
AUTHORS Rogers CI, Hinrichs T, Johnson LA, Zhao Y, Barolo S.
TITLE Direct Submission
JOURNAL
REFERENCE 3 (bases 1 to 14142)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 14142)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene 408
(1-2), 180-186 (2008)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(02-FEB-2007) Cell "
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..14142
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature complement(1..233)
/label=P element 3' end
/note="P element 3' end"
gene 242..4247
/gene="white"
/allele="+mC"
/label=white
protein_bind 4255..4379
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter 4404..4434
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
CDS 4488..5144
/label=CmR
/note="chloramphenicol acetyltransferase"
CDS 5489..5791
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(5835..5959)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
misc_feature 5965..6712
/label=spacer sequence includes partial KanR gene
/note="spacer sequence includes partial KanR gene"
regulatory 6713..6846
/label=Drosophila melanogaster Hsp70 minimal promoter
/note="Drosophila melanogaster Hsp70 minimal promoter"
/regulatory_class="promoter"
misc_feature 6920..7352
/gene="P transposase-lacZ-SV40t"
/label=P transposase leader sequence
/note="P transposase leader sequence"
CDS 7359..10412
/label=lacZ
/note="beta-galactosidase"
polyA_signal 10544..10678
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature complement(10797..11382)
/label=P element 5' end
rep_origin complement(11617..12205)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(12379..13236)
/label=AmpR
/note="beta-lactamase"
promoter complement(13237..13341)
/label=AmpR promoter
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