Ganesh-R1 vector (V009965)

Basic Vector Information

Vector Name:
Ganesh-R1
Antibiotic Resistance:
Ampicillin
Length:
11450 bp
Type:
Reporter vector
Replication origin:
ori
Source/Author:
Swanson CI, Hinrichs T, Johnson LA, Zhao Y, Barolo S.
Promoter:
lac UV5

Ganesh-R1 vector Vector Map

Ganesh-R111450 bp500100015002000250030003500400045005000550060006500700075008000850090009500100001050011000P element 3' endwhiteattR1lac UV5 promoterCmRccdBattR2spacer sequence includes partial KanR geneDrosophila melanogaster Hsp70 minimal promoterDsRed-ExpressDrosophila melanogaster tra gene NLSSV40 poly(A) signalP element 5' endoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

Ganesh-R1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_1014       11450 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector Ganesh-R1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   Swanson CI, Hinrichs T, Johnson LA, Zhao Y, Barolo S.
  TITLE     A directional recombination cloning system for restriction- and 
            ligation-free construction of GFP, DsRed, and lacZ transgenic 
            Drosophila reporters
  JOURNAL   Gene 408 (1-2), 180-186 (2008)
  PUBMED    18077106
REFERENCE   2  (bases 1 to 11450)
  AUTHORS   Rogers CI, Hinrichs T, Johnson LA, Zhao Y, Barolo S.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 11450)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11450)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 408 
            (1-2), 180-186 (2008)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2007) Cell "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     gene            242..4247
                     /gene="white"
                     /allele="+mC"
                     /label=white
     protein_bind    4255..4379
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        4404..4434
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             4488..5144
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             5489..5791
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(5835..5959)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    5965..6712
                     /label=spacer sequence includes partial KanR gene
                     /note="spacer sequence includes partial KanR gene"
     regulatory      6713..6846
                     /label=Drosophila melanogaster Hsp70 minimal promoter
                     /note="Drosophila melanogaster Hsp70 minimal promoter"
                     /regulatory_class="promoter"
     CDS             6920..7594
                     /label=DsRed-Express
                     /note="rapidly maturing tetrameric variant of DsRed
                     fluorescent protein (Bevis and Glick, 2002)"
     misc_feature    7598..7714
                     /gene="DsRed.T4-NLS-SV40t"
                     /label=Drosophila melanogaster tra gene NLS
                     /note="Drosophila melanogaster tra gene NLS"
     polyA_signal    7852..7986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(8105..8690)
                     /label=P element 5' end
     rep_origin      complement(8925..9513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9687..10544)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10545..10649)
                     /label=AmpR promoter

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