Ganesh-R1 vector (V009965)

LOCUS       40924_1014       11450 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector Ganesh-R1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   Swanson CI, Hinrichs T, Johnson LA, Zhao Y, Barolo S.
  TITLE     A directional recombination cloning system for restriction- and 
            ligation-free construction of GFP, DsRed, and lacZ transgenic 
            Drosophila reporters
  JOURNAL   Gene 408 (1-2), 180-186 (2008)
  PUBMED    18077106
REFERENCE   2  (bases 1 to 11450)
  AUTHORS   Rogers CI, Hinrichs T, Johnson LA, Zhao Y, Barolo S.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 11450)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11450)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 408 
            (1-2), 180-186 (2008)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2007) Cell "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     gene            242..4247
                     /gene="white"
                     /allele="+mC"
                     /label=white
     protein_bind    4255..4379
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        4404..4434
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             4488..5144
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             5489..5791
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(5835..5959)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    5965..6712
                     /label=spacer sequence includes partial KanR gene
                     /note="spacer sequence includes partial KanR gene"
     regulatory      6713..6846
                     /label=Drosophila melanogaster Hsp70 minimal promoter
                     /note="Drosophila melanogaster Hsp70 minimal promoter"
                     /regulatory_class="promoter"
     CDS             6920..7594
                     /label=DsRed-Express
                     /note="rapidly maturing tetrameric variant of DsRed
                     fluorescent protein (Bevis and Glick, 2002)"
     misc_feature    7598..7714
                     /gene="DsRed.T4-NLS-SV40t"
                     /label=Drosophila melanogaster tra gene NLS
                     /note="Drosophila melanogaster tra gene NLS"
     polyA_signal    7852..7986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(8105..8690)
                     /label=P element 5' end
     rep_origin      complement(8925..9513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9687..10544)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10545..10649)
                     /label=AmpR promoter