Basic Vector Information
Ganesh-R1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Ganesh-R1 vector Sequence
LOCUS 40924_1014 11450 bp DNA circular SYN 17-DEC-2018 DEFINITION Reporter vector Ganesh-R1, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11450) AUTHORS Swanson CI, Hinrichs T, Johnson LA, Zhao Y, Barolo S. TITLE A directional recombination cloning system for restriction- and ligation-free construction of GFP, DsRed, and lacZ transgenic Drosophila reporters JOURNAL Gene 408 (1-2), 180-186 (2008) PUBMED 18077106 REFERENCE 2 (bases 1 to 11450) AUTHORS Rogers CI, Hinrichs T, Johnson LA, Zhao Y, Barolo S. TITLE Direct Submission JOURNAL REFERENCE 3 (bases 1 to 11450) TITLE Direct Submission REFERENCE 4 (bases 1 to 11450) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene 408 (1-2), 180-186 (2008)" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-FEB-2007) Cell " COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..11450 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature complement(1..233) /label=P element 3' end /note="P element 3' end" gene 242..4247 /gene="white" /allele="+mC" /label=white protein_bind 4255..4379 /label=attR1 /note="recombination site for the Gateway(R) LR reaction" promoter 4404..4434 /label=lac UV5 promoter /note="E. coli lac promoter with an 'up' mutation" CDS 4488..5144 /label=CmR /note="chloramphenicol acetyltransferase" CDS 5489..5791 /label=ccdB /note="CcdB, a bacterial toxin that poisons DNA gyrase" protein_bind complement(5835..5959) /label=attR2 /note="recombination site for the Gateway(R) LR reaction" misc_feature 5965..6712 /label=spacer sequence includes partial KanR gene /note="spacer sequence includes partial KanR gene" regulatory 6713..6846 /label=Drosophila melanogaster Hsp70 minimal promoter /note="Drosophila melanogaster Hsp70 minimal promoter" /regulatory_class="promoter" CDS 6920..7594 /label=DsRed-Express /note="rapidly maturing tetrameric variant of DsRed fluorescent protein (Bevis and Glick, 2002)" misc_feature 7598..7714 /gene="DsRed.T4-NLS-SV40t" /label=Drosophila melanogaster tra gene NLS /note="Drosophila melanogaster tra gene NLS" polyA_signal 7852..7986 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" misc_feature complement(8105..8690) /label=P element 5' end rep_origin complement(8925..9513) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(9687..10544) /label=AmpR /note="beta-lactamase" promoter complement(10545..10649) /label=AmpR promoter
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