GAANTRY P donor TBS vector (V009969)

Basic Vector Information

      • Vector Name:
      • GAANTRY P donor TBS
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 8480 bp
      • Type:
      • Cloning vector
      • Replication origin:
      • ori
      • Source/Author:
      • Collier R, Thomson JG, Thilmony R.
      • Promoter:
      • sacB

GAANTRY P donor TBS vector Vector Map

GAANTRY P donor TBS8480 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400enhancer blocking insulator; derived from petunia; transformation booster sequence (TBS)A118 attPRB T-DNA repeatKanRM13 revlac operatorlac promoterCAP binding sitesacB promoterSacBoriAmpRAmpR promoterf1 oriM13 fwdTP901 attP

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

GAANTRY P donor TBS vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_989        8480 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GAANTRY P donor TBS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   Collier R, Thomson JG, Thilmony R.
  TITLE     GAANTRY, a versatile and robust Agrobacterium-based gene stacking 
            system generates high quality transgenic plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8480)
  AUTHORS   Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2017) Crop Improvement and Genetics research Unit,
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 8480)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8480)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-DEC-2017) Crop Improvement and Genetics research Unit, 
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..2018
                     /note="enhancer blocking insulator; derived from petunia; 
                     transformation booster sequence (TBS)"
                     /regulatory_class="insulator"
     misc_feature    2118..2173
                     /label=A118 attP
                     /note="A118 attP"
     misc_feature    2210..2234
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2566..3357
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(3680..3696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3704..3720)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3728..3758)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3773..3794)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4027..4472
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             4473..5891
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     rep_origin      complement(5992..6580)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6754..7611)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7612..7716)
                     /label=AmpR promoter
     rep_origin      complement(7742..8197)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8339..8355
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8364..8428
                     /label=TP901 attP
                     /note="TP901 attP"

This page is informational only.