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GAANTRY B helper vector (V009973#)

Basic Vector Information

Basic Vector Information
Vector Name GAANTRY B helper Antibiotic Resistance Ampicillin
Length 6338 bp Type Cloning vector
Source Collier R, Thomson JG, Thilmony R.

GAANTRY B helper vector Vector Map

f1 ori M13 fwd M13 fwd lac promoter ParA A118 T3 transcriptional terminator AmpR promoter lacI T7 terminator M13 rev lac operator lac promoter CAP binding site ColE1 origin of replication AmpR AmpR promoter

GAANTRY B helper vector Sequence

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Download GeneBank File(.gb)

LOCUS       Exported                6338 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GAANTRY B helper, complete sequence.
ACCESSION   MG687274
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Collier R, Thomson JG, Thilmony R.
  TITLE     GAANTRY, a versatile and robust Agrobacterium-based gene stacking 
            system generates high quality transgenic plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6338)
  AUTHORS   Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2017) Crop Improvement and Genetics research Unit,
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 6338)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Cloning vector GAANTRY B helper"
                     /mol_type="other DNA"
                     /db_xref="taxon:2201074"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     633..649
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      661..778
                     /regulatory_class="promoter"
                     /note="lac promoter"
     protein_bind    669..690
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        705..735
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    743..759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             779..1447
                     /codon_start=1
                     /gene="ParA"
                     /product="ParA"
                     /label=ParA
                     /note="small serine site-specific recombinase"
                     /protein_id="AWK49527.1"
                     /translation="MATREQQPEGRRLKVARIYLRASTDEQNLERQESLVAATRAAGYY
                     VAGIYREKASGARADRPELLRMIADLQPGEVVVAEKIDRISRLPLAEAERLVASIRAKG
                     AKLAVPGVVDLSELAAEANGVAKIVLESVQDMLLKLALQMARDDYEDRRERQRQGVQLA
                     KAAGRYTGRKRDAGMHDRIIALRSGGSSIAKTAKLVGCSPSQVKRVWAAWNAQQQNKRA
                     "
     gene            779..1447
                     /gene="ParA"
                     /label=ParA
     CDS             1455..2813
                     /codon_start=1
                     /gene="A118"
                     /product="A118"
                     /label=A118
                     /note="A118 large serine site-specific recombinase"
                     /protein_id="AWK49528.1"
                     /translation="MKAAIYIRVSTQEQVENYSIQAQTEKLTALCRSKDWDVYDIFIDG
                     GYSGSNMNRPALNEMLSKLHEIDAVVVYRLDRLSRSQRDTITLIEEYFLKNNVEFVSLS
                     ETLDTSSPFGRAMIGILSVFAQLERETIRDRMVMGKIKRIEAGLPLTTAKGRTFGYDVI
                     DTKLYINEEEAKQLQLIYDIFEEEQSITFLQKRLKKLGFKVRTYNRYNNWLTNDLYCGY
                     VSYKDKVHVKGIHEPIISEEQFYRVQEIFTRMGKNPNMNRDSASLLNNLVVCSKCGLGF
                     VHRRKDTMSRGKKYHYRYYSCKTYKHTHELEKCGNKIWRADKLEELIINRVNNYSFASR
                     NVDKEDELDSLNEKLKIEHAKKKRLFDLYINGSYEVSELDSMMNDIDAQINYYESQIEA
                     NEELKKNKKIQENLADLATVDFDSLEFREKQLYLKSLINKIYIDGEQVTIEWL"
     gene            1455..2813
                     /gene="A118"
                     /label=A118
     regulatory      2820..2868
                     /regulatory_class="terminator"
                     /note="T3 transcriptional terminator"
     promoter        2888..2992
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2993..4084
                     /codon_start=1
                     /gene="lacI"
                     /product="LacI repressor"
                     /label=lacI
                     /protein_id="AWK49529.1"
                     /translation="MVNVKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAM
                     AELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVER
                     SGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGD
                     WSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTED
                     SSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTA
                     SPRALADSLMQLARQVSRLESGQ"
     gene            2993..4084
                     /gene="lacI"
                     /label=lacI
     regulatory      4085..4131
                     /regulatory_class="terminator"
                     /note="T7 terminator"
     terminator      4085..4131
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(4189..4205)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4213..4229
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4237..4267)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4282..4303
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4591..5179)
                     /direction=LEFT
                     /label=ColE1 origin of replication
                     /note="ColE1 origin of replication"
     CDS             complement(5350..6210)
                     /codon_start=1
                     /gene="AmpR"
                     /product="AmpR"
                     /label=AmpR
                     /note="confers resistance to ampicillin"
                     /protein_id="AWK49530.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5350..6210)
                     /gene="AmpR"
                     /label=AmpR
     promoter        complement(6211..6315)
                     /gene="bla"
                     /label=AmpR promoter

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