Basic Vector Information
GAANTRY B donor tdTom vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
GAANTRY B donor tdTom vector Sequence
LOCUS 40924_959 8923 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector GAANTRY B donor tdTom, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8923) AUTHORS Collier R, Thomson JG, Thilmony R. TITLE GAANTRY, a versatile and robust Agrobacterium-based gene stacking system generates high quality transgenic plants JOURNAL Unpublished REFERENCE 2 (bases 1 to 8923) AUTHORS Thilmony R. TITLE Direct Submission JOURNAL Submitted (15-DEC-2017) Crop Improvement and Genetics research Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA REFERENCE 3 (bases 1 to 8923) TITLE Direct Submission REFERENCE 4 (bases 1 to 8923) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (15-DEC-2017) Crop Improvement and Genetics research Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..8923 /mol_type="other DNA" /organism="synthetic DNA construct" polyA_signal complement(38..212) /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" regulatory complement(218..446) /label=nos terminator /note="nos terminator" /regulatory_class="terminator" CDS complement(490..1917) /label=tdTomato /note="tandem dimeric (pseudo-monomeric) derivative of DsRed (Shaner et al., 2004)" promoter complement(2032..2708) /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" misc_feature 2937..2991 /label=TP901 attB /note="TP901 attB" misc_feature 3013..3037 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" promoter 3196..3224 /label=Pc promoter /note="class 1 integron promoter" CDS 3413..3943 /label=GmR /note="gentamycin acetyltransferase" misc_feature 4022..4127 /label=ParA MRS /note="ParA MRS" primer_bind complement(4150..4166) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(4174..4190) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4198..4228) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4243..4264) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 4497..4942 /label=sacB promoter /note="sacB promoter and control region" CDS 4943..6361 /label=SacB /note="secreted levansucrase that renders bacterial growth sensitive to sucrose" rep_origin complement(6462..7050) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7224..8081) /label=AmpR /note="beta-lactamase" promoter complement(8082..8186) /label=AmpR promoter rep_origin complement(8212..8667) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 8809..8825 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 8856..8911 /label=A118 attB /note="A118 attB"
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