GAANTRY B donor sul1 vector (V009976)

MSDS

Basic Vector Information

Vector Name:: GAANTRY B donor sul1

Antibiotic Resistance:: Ampicillin

Length:: 8026 bp

Type:: Cloning vector

Replication origin:: ori

Host:: Plants

Source/Author:: Collier R, Thomson JG, Thilmony R.

Promoter:: CaMV 35S (enhanced)

GAANTRY B donor sul1 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

GAANTRY B donor sul1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_954        8026 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GAANTRY B donor sul1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Collier R, Thomson JG, Thilmony R.
  TITLE     GAANTRY, a versatile and robust Agrobacterium-based gene stacking 
            system generates high quality transgenic plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8026)
  AUTHORS   Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2017) Crop Improvement and Genetics research Unit,
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 8026)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8026)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-DEC-2017) Crop Improvement and Genetics research Unit, 
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(1..160)
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     CDS             complement(218..1054)
                     /gene="sulI"
                     /label=sulI
                     /note="Dihydropteroate synthase type-1 from Escherichia
                     coli. Accession#: P0C002"
     promoter        complement(1163..1834)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1959..2013
                     /label=TP901 attB
                     /note="TP901 attB"
     misc_feature    2035..2059
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2218..2246
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2435..2965
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     misc_feature    3044..3149
                     /label=ParA MRS
                     /note="ParA MRS"
     primer_bind     complement(3172..3188)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3196..3212)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3220..3250)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3265..3286)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3519..3964
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             3965..5383
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     rep_origin      complement(5484..6072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6246..7103)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7104..7208)
                     /label=AmpR promoter
     rep_origin      complement(7234..7689)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7831..7847
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7878..7933
                     /label=A118 attB
                     /note="A118 attB"

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