GAANTRY B donor sul1 vector (V009976)

LOCUS       V009976                 8026 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009976
VERSION     V009976
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Collier R, Thomson JG, Thilmony R.
  TITLE     GAANTRY, a versatile and robust Agrobacterium-based gene stacking
            system generates high quality transgenic plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8026)
  AUTHORS   Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2017) Crop Improvement and Genetics research Unit,
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 8026)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8026)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-DEC-2017) Crop Improvement and Genetics research Unit,
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(1..160)
                     /label="CaMV 35S terminator"
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     CDS             complement(218..1054)
                     /gene="sulI"
                     /label="Dihydropteroate synthase type-1"
                     /note="Dihydropteroate synthase type-1 from Escherichia
                     coli. Accession#: P0C002"
     promoter        complement(1163..1834)
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1959..2013
                     /label="TP901 attB"
                     /note="TP901 attB"
     misc_feature    2035..2059
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2218..2246
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             2435..2965
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     misc_feature    3044..3149
                     /label="ParA MRS"
                     /note="ParA MRS"
     primer_bind     complement(3172..3188)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3196..3212)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3220..3250)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3265..3286)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3519..3964
                     /label="sacB promoter"
                     /note="sacB promoter and control region"
     CDS             3965..5383
                     /label="SacB"
                     /note="secreted levansucrase that renders bacterial growth
                     sensitive to sucrose"
     rep_origin      complement(5484..6072)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6246..7103)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7104..7208)
                     /label="AmpR promoter"
     rep_origin      complement(7234..7689)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7831..7847
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    7878..7933
                     /label="A118 attB"
                     /note="A118 attB"