Basic Vector Information
GAANTRY B donor MoroMYB vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
GAANTRY B donor MoroMYB vector Sequence
LOCUS Exported 9281 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector GAANTRY B donor MoroMYB, complete sequence. ACCESSION MG687282 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9281) AUTHORS Collier R, Thomson JG, Thilmony R. TITLE GAANTRY, a versatile and robust Agrobacterium-based gene stacking system generates high quality transgenic plants JOURNAL Unpublished REFERENCE 2 (bases 1 to 9281) AUTHORS Thilmony R. TITLE Direct Submission JOURNAL Submitted (15-DEC-2017) Crop Improvement and Genetics research Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA REFERENCE 3 (bases 1 to 9281) AUTHORS . TITLE Direct Submission COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..9281 /organism="Cloning vector GAANTRY B donor MoroMYB" /mol_type="other DNA" /db_xref="taxon:2201070" regulatory complement(7..218) /regulatory_class="terminator" /note="CaMV 35S terminator" CDS complement(join(232..772,1568..1697,1795..1912)) /codon_start=1 /product="Moro MYBA" /label=Moro MYBA /note="derived from Citrus sinensis" /protein_id="AWK49560.1" /translation="MADSLGVRKGAWTGEEDDLLRKCIEKYGEAKWHQVPLRAGLHRCR KSCRLRWLNYLNPNIKRGEFAADEVDLILRLHKLLGNRWSLIVGRLPGRTANDVKNFWN THLRKKVDKCCKNNKEMKAKAEKVEKINIIKPQPRTFAKNSQWLKGKGMTSNNLQLGDY NLGKQSTPSDHHHHHQQQQENETESVWWESFLFGDELDQQGISSSLSRPEEESTTANIF AEKSPVVTKVTENRVIEAGQSCPTDDFAFDAELWDLLNAK" regulatory complement(1924..3163) /regulatory_class="promoter" /note="Arabidopsis HotHead (HTH) promoter" misc_feature 3224..3278 /label=TP901 attB /note="TP901 attB" misc_feature complement(3300..3324) /label=Agrobacterium T-DNA right border /note="Agrobacterium T-DNA right border" promoter 3483..3511 /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter" misc_feature 3552..3657 /label=ParA MRS /note="ParA MRS" CDS 3700..4233 /codon_start=1 /gene="aacC1" /product="gentamicin acetyltransferase" /label=aacC1 /note="confers resistance to gentamicin" /protein_id="AWK49561.1" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" gene 3700..4233 /gene="aacC1" /label=aacC1 misc_feature 4309..4414 /label=ParA MRS /note="ParA MRS" primer_bind complement(4437..4453) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 4461..4477 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4485..4515) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4530..4551 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 4784..5229 /gene="sacR" /label=sacB promoter /note="sacB promoter and control region" CDS 5230..6651 /codon_start=1 /gene="sacB" /product="levansucrase" /label=sacB /note="confers sensitivity to sucrose" /protein_id="AWK49562.1" /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV VKDSILEQGQLTVNK" gene 5230..6651 /gene="sacB" /label=sacB rep_origin complement(6749..7337) /direction=LEFT /label=ColE1 origin of replication /note="ColE1 origin of replication" misc_feature 7508..8368 /label=A118 attB /note="A118 attB" CDS complement(7508..8368) /codon_start=1 /gene="AmpR" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin" /protein_id="AWK49563.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" gene complement(7508..8368) /gene="AmpR" /label=AmpR promoter complement(8369..8473) /gene="bla" /label=AmpR promoter rep_origin complement(8499..8954) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 9096..9112 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants"
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