GAANTRY B donor GUS vector (V009978)

LOCUS       40924_944       10366 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GAANTRY B donor GUS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10366)
  AUTHORS   Collier R, Thomson JG, Thilmony R.
  TITLE     GAANTRY, a versatile and robust Agrobacterium-based gene stacking 
            system generates high quality transgenic plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10366)
  AUTHORS   Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2017) Crop Improvement and Genetics research Unit,
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 10366)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10366)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-DEC-2017) Crop Improvement and Genetics research Unit, 
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(29..281)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(356..2164)
                     /label=GUS
                     /note="beta-glucuronidase"
     CDS             complement(2177..2197)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     regulatory      complement(2216..4312)
                     /label=Pinus radiata ubiquitin promoter and 5' intron
                     /note="Pinus radiata ubiquitin promoter and 5' intron"
                     /regulatory_class="promoter"
     primer_bind     complement(4327..4343)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4392..4446
                     /label=TP901 attB
                     /note="TP901 attB"
     misc_feature    4468..4492
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        4651..4679
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             4868..5398
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     misc_feature    5477..5582
                     /label=ParA MRS
                     /note="ParA MRS"
     primer_bind     complement(5605..5621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5629..5645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5653..5683)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5698..5719)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5952..6397
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             6398..7816
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     rep_origin      complement(7917..8505)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8679..9536)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9537..9641)
                     /label=AmpR promoter
     rep_origin      complement(9667..10122)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     10264..10280
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    10311..10366
                     /label=A118 attB
                     /note="A118 attB"