Basic Vector Information
- Vector Name:
- GAANTRY B donor eGFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8899 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Collier R, Thomson JG, Thilmony R.
- Promoter:
- sacB
GAANTRY B donor eGFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
GAANTRY B donor eGFP vector Sequence
LOCUS 40924_939 8899 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector GAANTRY B donor eGFP, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8899)
AUTHORS Collier R, Thomson JG, Thilmony R.
TITLE GAANTRY, a versatile and robust Agrobacterium-based gene stacking
system generates high quality transgenic plants
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8899)
AUTHORS Thilmony R.
TITLE Direct Submission
JOURNAL Submitted (15-DEC-2017) Crop Improvement and Genetics research Unit,
USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE 3 (bases 1 to 8899)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8899)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(15-DEC-2017) Crop Improvement and Genetics research Unit,
USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..8899
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory complement(8..219)
/label=CaMV 35S terminator
/note="CaMV 35S terminator"
/regulatory_class="terminator"
CDS complement(238..954)
/label=EGFP
/note="enhanced GFP"
CDS complement(959..1188)
/codon_start=1
/gene="ubq"
/product="ubiquitin monomer"
/label=ubq
/note="ubiquitin monomer confers enhanced expression in
translational fusions"
/protein_id="AWK49545.1"
/translation="MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIF
AGKQLEDGRTLADYNIQKESTLHLVLRLRGG"
gene complement(959..1188)
/gene="ubq"
/label=ubq
regulatory complement(1189..2907)
/label=potato ubiquitin 7 promoter and 5' intron
/note="potato ubiquitin 7 promoter and 5' intron"
/regulatory_class="promoter"
protein_bind 2796..2807
/label=ZF binding site
/bound_moiety="CCR5TC zinc-finger domain"
/note="target sequence for the CCR5TC zinc-finger domain
from the CCR5-224 (+) zinc-finger nuclease (Perez et al.,
2008; Gross et al., 2013)"
misc_feature 2925..2979
/label=TP901 attB
/note="TP901 attB"
misc_feature 3001..3025
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
promoter 3184..3212
/label=Pc promoter
/note="class 1 integron promoter"
CDS 3401..3931
/label=GmR
/note="gentamycin acetyltransferase"
misc_feature 4010..4115
/label=ParA MRS
/note="ParA MRS"
primer_bind complement(4138..4154)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4162..4178)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4186..4216)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(4231..4252)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4485..4930
/label=sacB promoter
/note="sacB promoter and control region"
CDS 4931..6349
/label=SacB
/note="secreted levansucrase that renders bacterial growth
sensitive to sucrose"
rep_origin complement(6450..7038)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7212..8069)
/label=AmpR
/note="beta-lactamase"
promoter complement(8070..8174)
/label=AmpR promoter
rep_origin complement(8200..8655)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 8797..8813
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 8844..8899
/label=A118 attB
/note="A118 attB"
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