full-length T7 TNAP vector (V009982)

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LOCUS       V009982                 4969 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009982
VERSION     V009982
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Han T, Chen Q, Liu H.
  TITLE     Engineered photoactivatable genetic switches based on the bacterium
            phage T7 RNA polymerase
  JOURNAL   ACS Synth Biol (2016) In press
   PUBMED   27794600
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Han T, Chen Q, Liu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2016) School of Life Sciences, University of
            Science and Technology of China, University of Science and
            Technology of China, No.96, JinZhai Road Baohe District,Hefei,Anhui,
            230026,P.R.China, Hefei, Anhui 230026, China
REFERENCE   3  (bases 1 to 4969)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4969)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. Vector NTI Advance (TM) 11.0
            Assembly Name         :: full-length T7 RNAP
            Coverage              :: 2323bp
            Sequencing Technology :: 454
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
            Biol (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-OCT-2016) School of Life Sciences, University of Science and
            Technology of China, University of Science and Technology of China,
            No.96, JinZhai Road Baohe District,Hefei,Anhui, 230026,P.R.China,
            Hefei, Anhui 230026, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..116)
                     /label="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     regulatory      125..162
                     /regulatory_class="promoter"
     regulatory      179..190
                     /regulatory_class="ribosome_binding_site"
     RBS             179..190
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             197..2845
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     regulatory      2873..2906
                     /regulatory_class="terminator"
     misc_feature    2906..2926
                     /label="BioBrick suffix"
                     /note="universal suffix for all parts"
     terminator      2927..2984
                     /label="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(3179..3767)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4003..4815)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"