FMDV-2A-def vector (V009984)

Basic Vector Information

      • Vector Name:
      • FMDV-2A-def
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 14325 bp
      • Type:
      • YFV replicon vector
      • Replication origin:
      • p15A ori
      • Source/Author:
      • Shustov AV, Frolov IV.

FMDV-2A-def vector Vector Map

FMDV-2A-def14325 bp70014002100280035004200490056006300700077008400910098001050011200119001260013300140005'UTR of Yellow fever virus 17Dfirst 25 aa of Yellow fever virus 17D capsid proteinEGFP2A protease of Foot-and-Mouth Disease VirusGenome polyprotein from Yellow fever virus (strain 17D vaccine). Accession#: P033143'UTR of Yellow fever virus 17DSV40 poly(A) signalp15A oriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

FMDV-2A-def vector Sequence

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Download GeneBank File(.gb)

LOCUS       40924_850       14325 bp DNA     circular SYN 17-DEC-2018
DEFINITION  YFV replicon vector FMDV-2A-def, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14325)
  AUTHORS   Shustov AV, Frolov IV.
  TITLE     Trans-packaging of Yellow Fever virus replicons: an approach for 
            flaviviral antigen production
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14325)
  AUTHORS   Shustov AV, Frolov IV.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2005) Department of Microbiology and Immunology, 
            University of Texas Medical Branch, 301 University Boulevard, 
            Galveston, TX 77550-1019, USA
REFERENCE   3  (bases 1 to 14325)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14325)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-DEC-2005) Department of Microbiology and Immunology, University 
            of Texas Medical Branch, 301 University Boulevard, Galveston, TX 
            77550-1019, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2..197
                     /mol_type="other DNA"
                     /db_xref="taxon:11090"
                     /organism="Yellow fever virus 17D"
     misc_feature    2..119
                     /label=5'UTR of Yellow fever virus 17D
                     /note="5'UTR of Yellow fever virus 17D"
     misc_feature    120..194
                     /note="first 25 aa of Yellow fever virus 17D capsid
                     protein"
     CDS             198..914
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    918..968
                     /label=2A protease of Foot-and-Mouth Disease Virus
                     /note="2A protease of Foot-and-Mouth Disease Virus"
     CDS             969..10901
                     /note="Genome polyprotein from Yellow fever virus (strain
                     17D vaccine). Accession#: P03314"
     misc_feature    10905..11412
                     /label=3'UTR of Yellow fever virus 17D
                     /note="3'UTR of Yellow fever virus 17D"
     polyA_signal    11622..11703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(12367..12911)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(13079..13936)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(13937..14041)
                     /label=AmpR promoter

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