Basic Vector Information
FMDV-2A-def vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
FMDV-2A-def vector Sequence
LOCUS 40924_850 14325 bp DNA circular SYN 17-DEC-2018 DEFINITION YFV replicon vector FMDV-2A-def, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14325) AUTHORS Shustov AV, Frolov IV. TITLE Trans-packaging of Yellow Fever virus replicons: an approach for flaviviral antigen production JOURNAL Unpublished REFERENCE 2 (bases 1 to 14325) AUTHORS Shustov AV, Frolov IV. TITLE Direct Submission JOURNAL Submitted (10-DEC-2005) Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77550-1019, USA REFERENCE 3 (bases 1 to 14325) TITLE Direct Submission REFERENCE 4 (bases 1 to 14325) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (10-DEC-2005) Department of Microbiology and Immunology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77550-1019, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..14325 /mol_type="other DNA" /organism="synthetic DNA construct" source 2..197 /mol_type="other DNA" /db_xref="taxon:11090" /organism="Yellow fever virus 17D" misc_feature 2..119 /label=5'UTR of Yellow fever virus 17D /note="5'UTR of Yellow fever virus 17D" misc_feature 120..194 /note="first 25 aa of Yellow fever virus 17D capsid protein" CDS 198..914 /label=EGFP /note="enhanced GFP" misc_feature 918..968 /label=2A protease of Foot-and-Mouth Disease Virus /note="2A protease of Foot-and-Mouth Disease Virus" CDS 969..10901 /note="Genome polyprotein from Yellow fever virus (strain 17D vaccine). Accession#: P03314" misc_feature 10905..11412 /label=3'UTR of Yellow fever virus 17D /note="3'UTR of Yellow fever virus 17D" polyA_signal 11622..11703 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(12367..12911) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." CDS complement(13079..13936) /label=AmpR /note="beta-lactamase" promoter complement(13937..14041) /label=AmpR promoter
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