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EXP4(+) vector (V009993)

Basic Vector Information

      • Vector Name:
      • EXP4(+)
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 6724 bp
      • Type:
      • Cloning vector
      • Replication origin:
      • ori
      • Source/Author:
      • Meima ME, Weening KE, Schaap P.

EXP4(+) vector Vector Map

EXP4(+)6724 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600multipe cloning site2H3 terminatorNeoR/KanRAmpR promoterAmpRoriactin15 promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

EXP4(+) vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_800        6724 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector EXP4(+), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Meima ME, Weening KE, Schaap P.
  TITLE     Vectors for expression of proteins with single or combinatorial 
            fluorescent protein and tandem affinity purification tags in 
            Dictyostelium
  JOURNAL   Protein Expr. Purif. 53 (2), 283-288 (2007)
  PUBMED    17296313
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Meima ME, Weening KE, Schaap P.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2006) School of Life Sciences, University of 
            Dundee, Dow Street, Dundee DD1 5EH, United Kingdom
REFERENCE   3  (bases 1 to 6724)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6724)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr. Purif."; date: "2007"; volume: "53"; issue: "2"; pages: 
            "283-288"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-SEP-2006) School of Life Sciences, University of Dundee, Dow 
            Street, Dundee DD1 5EH, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..30
                     /label=multipe cloning site
                     /note="multipe cloning site"
     regulatory      42..1022
                     /label=2H3 terminator
                     /note="2H3 terminator"
                     /regulatory_class="terminator"
     CDS             1791..2582
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        3475..3579
                     /label=AmpR promoter
     CDS             3580..4437
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4611..5199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      6416..6724
                     /label=actin15 promoter
                     /note="actin15 promoter"
                     /regulatory_class="promoter"

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