DIO-SynaptoTag vector (V010008)

LOCUS       40924_670        7171 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector DIO-SynaptoTag, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Zhou M, Liu Z, Melin MD, Ng YH, Xu W, Sudhof TC.
  TITLE     A central amygdala to zona incerta projection is required for 
            acquisition and remote recall of conditioned fear memory
  JOURNAL   Nat. Neurosci. 21 (11), 1515-1519 (2018)
  PUBMED    30349111
REFERENCE   2  (bases 1 to 7171)
  AUTHORS   Zhou M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2018) Molecular and Cellular Physiology, Stanford 
            University, 265 Campus Drive, Room G1065, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 7171)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7171)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Neurosci."; date: "2018"; volume: "21"; issue: "11"; pages: 
            "1515-1519"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-SEP-2018) Molecular and Cellular Physiology, Stanford 
            University, 265 Campus Drive, Room G1065, Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     promoter        177..624
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    633..666
                     /label=lox2272
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    729..762
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1129..1845)
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    complement(1862..2419)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             complement(2428..3132)
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     regulatory      3138..3147
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3148..3181)
                     /label=lox2272
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(3252..3285)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3298..3886
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    3918..4394
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   4434..4574
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4649..5104
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5386..5490
                     /label=AmpR promoter
     CDS             5491..6348
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6522..7110
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"