DIO-SynaptoTag vector (V010008)

Basic Vector Information

      • Vector Name:
      • DIO-SynaptoTag
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 7171 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Zhou M, Liu Z, Melin MD, Ng YH, Xu W, Sudhof TC.

DIO-SynaptoTag vector Vector Map

DIO-SynaptoTag7171 bp30060090012001500180021002400270030003300360039004200450048005100540057006000630066006900AAV2 ITRderived from synapsinloxp2272loxpEGFP-Syb2IRESmCherryvertebrate consensus sequence for strong initiation of translation (Kozak, 1987)loxp2272loxpWPREhGH poly(A) signalAAV2 ITRf1 oriAmpR promoterAmpRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

DIO-SynaptoTag vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                7171 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector DIO-SynaptoTag, complete sequence.
ACCESSION   MH883617
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Zhou M, Liu Z, Melin MD, Ng YH, Xu W, Sudhof TC.
  TITLE     A central amygdala to zona incerta projection is required for 
            acquisition and remote recall of conditioned fear memory
  JOURNAL   Nat. Neurosci. 21 (11), 1515-1519 (2018)
  PUBMED    30349111
REFERENCE   2  (bases 1 to 7171)
  AUTHORS   Zhou M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2018) Molecular and Cellular Physiology, Stanford 
            University, 265 Campus Drive, Room G1065, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 7171)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="Cloning vector DIO-SynaptoTag"
                     /mol_type="other DNA"
                     /db_xref="taxon:2484859"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     regulatory      156..626
                     /regulatory_class="promoter"
                     /note="derived from synapsin"
     promoter        177..624
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_recomb     633..666
                     /label=loxp2272
                     /note="loxp2272"
     protein_bind    complement(633..666)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     misc_recomb     729..762
                     /label=loxp
                     /note="loxp"
     protein_bind    complement(729..762)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             complement(769..1845)
                     /codon_start=1
                     /product="EGFP-Syb2"
                     /label=EGFP-Syb2
                     /protein_id="AYP25870.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKGGGGSATAATVPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQV
                     DEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMII
                     LGVICAIILIIIIVYFST"
     CDS             complement(1129..1845)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      complement(1846..2418)
                     /regulatory_class="transcriptional_cis_regulatory_region"
                     /note="IRES"
     CDS             complement(2425..3132)
                     /codon_start=1
                     /product="mCherry"
                     /label=mCherry
                     /protein_id="AYP25871.1"
                     /translation="MSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     regulatory      3138..3147
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_recomb     complement(3148..3181)
                     /label=loxp2272
                     /note="loxp2272"
     protein_bind    3148..3181
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     misc_recomb     complement(3252..3285)
                     /label=loxp
                     /note="loxp"
     protein_bind    3252..3285
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    3298..3886
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3769..3780)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3918..4394
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   4434..4574
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4649..5104
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5386..5490
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5491..6351
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6522..7110
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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