DIO-SynaptoTag vector (V010008)

Basic Vector Information

      • Vector Name:
      • DIO-SynaptoTag
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 7171 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Zhou M, Liu Z, Melin MD, Ng YH, Xu W, Sudhof TC.

DIO-SynaptoTag vector Vector Map

DIO-SynaptoTag7171 bp30060090012001500180021002400270030003300360039004200450048005100540057006000630066006900AAV2 ITRhSyn promoterlox2272loxPEGFPIRESmCherryvertebrate consensus sequence for strong initiation of translation (Kozak, 1987)lox2272loxPWPREhGH poly(A) signalAAV2 ITRf1 oriAmpR promoterAmpRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

DIO-SynaptoTag vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_670        7171 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector DIO-SynaptoTag, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Zhou M, Liu Z, Melin MD, Ng YH, Xu W, Sudhof TC.
  TITLE     A central amygdala to zona incerta projection is required for 
            acquisition and remote recall of conditioned fear memory
  JOURNAL   Nat. Neurosci. 21 (11), 1515-1519 (2018)
  PUBMED    30349111
REFERENCE   2  (bases 1 to 7171)
  AUTHORS   Zhou M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2018) Molecular and Cellular Physiology, Stanford 
            University, 265 Campus Drive, Room G1065, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 7171)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7171)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Neurosci."; date: "2018"; volume: "21"; issue: "11"; pages: 
            "1515-1519"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-SEP-2018) Molecular and Cellular Physiology, Stanford 
            University, 265 Campus Drive, Room G1065, Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     promoter        177..624
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    633..666
                     /label=lox2272
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    729..762
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1129..1845)
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    complement(1862..2419)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             complement(2428..3132)
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     regulatory      3138..3147
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3148..3181)
                     /label=lox2272
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(3252..3285)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3298..3886
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    3918..4394
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   4434..4574
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4649..5104
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5386..5490
                     /label=AmpR promoter
     CDS             5491..6348
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6522..7110
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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